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Colloidal features of softwood galactoglucomannans-rich extract

DOI: 10.1016/j.carbpol.2020.116368 DOI Help

Authors: Mamata Bhattarai (University of Helsinki) , Irina Sulaeva (University of Natural Resources and Life Sciences) , Leena Pitkänen (University of Helsinki) , Inkeri Kontro (University of Helsinki) , Maija Tenkanen (University of Helsinki) , Antje Potthast (University of Natural Resources and Life Sciences) , Kirsi S. Mikkonen (University of Helsinki)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Carbohydrate Polymers

State: Published (Approved)
Published: April 2020

Open Access Open Access

Abstract: Development of a sustainable bioeconomy requires valorization of renewable resources, such as wood hemicelluloses. The intra- and inter-molecular associations of hemicelluloses within themselves or with other wood components can result in complex macromolecular features. These features exhibit functionality as hydrocolloids, however macromolecular characterization of these heterogeneous materials are challenging using conventional techniques such as size-exclusion chromatography. We studied galactoglucomannans (GGM) -rich softwood extracts at two grades of purity—as crude extract and after ethanol-precipitation. Asymmetrical flow field-flow fractionation (AF4) was optimized and utilized to fractionate size classes in GGM extracts, and subsequent characterization was performed with light scattering and microscopy techniques. Both GGM extracts contained polysaccharides of around 10,000 g/mol molar mass, and colloidal assemblies and/or particles in sub-micron size range. The optimized AF4 method facilitates the characterization of complex biomass-derived carbohydrates without pre-fractionation, and provides valuable understanding of their unique macromolecular features for their future application in food, pharmaceuticals, and cosmetics.

Journal Keywords: asymmetrical flow field-flow fractionation; wood hemicelluloses; galactoglucomannans; particles; aggregates

Subject Areas: Biology and Bio-materials, Chemistry, Environment

Instruments: B21-High Throughput SAXS


Discipline Tags:

Biomaterials Chemistry Earth Sciences & Environment Material Sciences Organic Chemistry Polymer Science Soft condensed matter physics

Technical Tags:

Scattering Small Angle X-ray Scattering (SAXS)