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Low-dose phase retrieval of biological specimens using cryo-electron ptychography
DOI:
10.1038/s41467-020-16391-6
Authors:
Liqi
Zhou
(Nanjing University)
,
Jingdong
Song
(Chinese Center for Disease Control and Prevention)
,
Judy S.
Kim
(University of Oxford; Diamond Light Source; The Rosalind Franklin Institute)
,
Xudong
Pei
(Nanjing University)
,
Chen
Huang
(University of Oxford; Diamond Light Source)
,
Mark
Boyce
(Wellcome Trust Centre for Human Genetics, University of Oxford)
,
Luiza
Mendonca
(Wellcome Trust Centre for Human Genetics, University of Oxford)
,
Daniel
Clare
(Diamond Light Source)
,
Alistair
Siebert
(Diamond Light Source)
,
Christopher
Allen
(University of Oxford; Diamond Light Source Ltd)
,
Emanuela
Liberti
(University of Oxford; Diamond Light Source)
,
David
Stuart
(Wellcome Trust Centre for Human Genetics, University of Oxford; Diamond Light Source)
,
Xiaoqing
Pan
(University of California)
,
Peter
Nellist
(University of Oxford)
,
Peijun
Zhang
(Wellcome Trust Centre for Human Genetics, University of Oxford; Diamond Light Source)
,
Angus
Kirkland
(University of Oxford; Diamond Light Source; The Rosalind Franklin Institute)
,
Peng
Wang
(Nanjing University)
Co-authored by industrial partner:
No
Type:
Journal Paper
Journal:
Nature Communications
, VOL 11
State:
Published (Approved)
Published:
June 2020
Diamond Proposal Number(s):
19243
,
20431
,
20961
,
22317

Abstract: Cryo-electron microscopy is an essential tool for high-resolution structural studies of biological systems. This method relies on the use of phase contrast imaging at high defocus to improve information transfer at low spatial frequencies at the expense of higher spatial frequencies. Here we demonstrate that electron ptychography can recover the phase of the specimen with continuous information transfer across a wide range of the spatial frequency spectrum, with improved transfer at lower spatial frequencies, and as such is more efficient for phase recovery than conventional phase contrast imaging. We further show that the method can be used to study frozen-hydrated specimens of rotavirus double-layered particles and HIV-1 virus-like particles under low-dose conditions (5.7 e/Å2) and heterogeneous objects in an Adenovirus-infected cell over large fields of view (1.14 × 1.14 μm), thus making it suitable for studies of many biologically important structures.
Journal Keywords: Electron microscopy; Ptychography; TEM; Virus; CryoEM
Diamond Keywords: Viruses
Subject Areas:
Physics,
Biology and Bio-materials,
Technique Development
Diamond Offline Facilities:
Electron Physical Sciences Imaging Centre (ePSIC)
Instruments:
E02-JEM ARM 300CF
Added On:
08/06/2020 11:33
Documents:
s41467-020-16391-6.pdf
Discipline Tags:
Pathogens
Infectious Diseases
Health & Wellbeing
Technique Development - Life Sciences & Biotech
Life Sciences & Biotech
Technical Tags:
Imaging
Microscopy
Coherent Diffraction Imaging (CDI)
Ptychography
Electron Microscopy (EM)
Transmission Electron Microscopy (TEM)