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Crystal structures of the GH18 domain of the bifunctional peroxiredoxin–chitinase CotE from Clostridium difficile

DOI: 10.1107/S2053230X20006147 DOI Help

Authors: Jean L. Whittingham (University of York) , Shumpei Hanai (Osaka University) , James A. Brannigan (University of York) , William T. Ferreira (Royal Holloway University of London) , Eleanor J. Dodson (University of York) , Johan Turkenburg (University of York) , Jared Cartwright (University of York) , Simon M. Cutting (Royal Holloway University of London) , Anthony Wilkinson (University of York)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section F Structural Biology Communications , VOL 76 , PAGES 241 - 249

State: Published (Approved)
Published: June 2020
Diamond Proposal Number(s): 9948

Open Access Open Access

Abstract: CotE is a coat protein that is present in the spores of Clostridium difficile, an obligate anaerobic bacterium and a pathogen that is a leading cause of antibiotic-associated diarrhoea in hospital patients. Spores serve as the agents of disease transmission, and CotE has been implicated in their attachment to the gut epithelium and subsequent colonization of the host. CotE consists of an N-terminal peroxiredoxin domain and a C-terminal chitinase domain. Here, a C-terminal fragment of CotE comprising residues 349–712 has been crystallized and its structure has been determined to reveal a core eight-stranded β-barrel fold with a neighbouring subdomain containing a five-stranded β-sheet. A prominent groove running across the top of the barrel is lined by residues that are conserved in family 18 glycosyl hydrolases and which participate in catalysis. Electron density identified in the groove defines the pentapeptide Gly-Pro-Ala-Met-Lys derived from the N-terminus of the protein following proteolytic cleavage to remove an affinity-purification tag. These observations suggest the possibility of designing peptidomimetics to block C. difficile transmission.

Journal Keywords: Clostridium difficile; spores; CotE; glycosyl hydrolase; 3D domain swapping

Subject Areas: Biology and Bio-materials

Instruments: I02-Macromolecular Crystallography , I03-Macromolecular Crystallography