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X-ray diffraction imaging of corneal ultrastructure

DOI: 10.1007/978-1-0716-0599-8_16 DOI Help

Authors: Keith Meek (Cardiff University) , Andrew J. Quantock (Cardiff University) , Sally Hayes (University of Cardiff) , James Bell (Cardiff University)
Co-authored by industrial partner: No

Type: Book Chapter

State: Published (Approved)
Published: June 2020

Abstract: X-ray scattering enables the structure of collagen-rich tissues, such as the cornea, to be examined at both the molecular and fibrillar level. The high-intensity X-rays available at synchrotron radiation sources, coupled with minimal sample preparation requirements, facilitates the rapid generation of high-quality X-ray scattering data from corneal tissue at a close-to-physiological state of hydration. Analysis of resulting X-ray scatter patterns allows one to quantify numerous structural parameters relating to the average diameter, lateral arrangement and alignment of collagen fibrils within the cornea, as well as the axial and lateral arrangements of collagen molecules within the fibrils. Here we describe the typical experimental setup and considerations involved in the collection of X-ray scattering data from corneal tissue.

Journal Keywords: X-ray; Cornea; Synchrotron; Collagen hydrogels

Subject Areas: Biology and Bio-materials

Instruments: I22-Small angle scattering & Diffraction

Other Facilities: 40XU at SPring8; X12B at the National Synchrotron Light Source

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