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Ovine bone morphology and deformation analysis using synchrotron X-ray imaging and scattering

DOI: 10.3390/qubs4030029 DOI Help

Authors: Eugene S. Statnik (Skoltech; National University of Science and Technology MISiS) , Alexey I. Salimon (National University of Science and Technology MISiS; Skoltech) , Cyril Besnard (University of Oxford) , Jingwei Chen (University of Oxford) , Zifan Wang (University of Oxford) , Thomas Moxham (University of Oxford; Diamond Light Source) , Igor P. Dolbnya (Diamond Light Source) , Alexander Korsunsky (Skoltech; University of Oxford; Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Quantum Beam Science , VOL 4

State: Published (Approved)
Published: August 2020
Diamond Proposal Number(s): 21419

Open Access Open Access

Abstract: Bone is a natural hierarchical composite tissue incorporating hard mineral nano-crystals of hydroxyapatite (HAp) and organic binding material containing elastic collagen fibers. In the study, we investigated the structure and deformation of ovine bone by the combination of high-energy synchrotron X-ray tomographic imaging and scattering. X-ray experiments were performed prior to and under three-point bending loading by using a specially developed in situ load cell constructed from aluminium alloy frame, fast-drying epoxy resin for sample fixation, and a titanium bolt for contact loading. Firstly, multiple radiographic projection images were acquired and tomographic reconstruction was performed using SAVU software, following segmentation using Avizo. Secondly, Wide Angle X-ray Scattering (WAXS) and Small Angle X-ray Scattering (SAXS) 2D scattering patterns were collected from HAp and collagen. Both sample shape and deformation affect the observed scattering. Novel combined tomographic and diffraction analysis presented below paves the way for advanced characterization of complex shape samples using the Dual Imaging and Diffraction (DIAD) paradigm.

Journal Keywords: residual stress/strain; ovine bone; synchrotron X-ray diffraction; SAVU tomography reconstruction; Avizo segmentation

Subject Areas: Biology and Bio-materials


Instruments: B16-Test Beamline

Documents:
qubs-04-00029.pdf