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Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown

DOI: 10.1038/s41467-020-17847-5 DOI Help

Authors: Lucy I. Crouch (Newcastle University) , Marcelo V. Liberato (Universidade de Sorocaba) , Paulina A. Urbanowicz (Ludger Ltd) , Arnaud Basle (Newcastle University) , Christopher A. Lamb (Newcastle upon Tyne Hospitals NHS Foundation Trust) , Christopher J. Stewart (Newcastle University) , Katie Cooke (Newcastle University) , Mary Doona (Newcastle upon Tyne Hospitals NHS Foundation Trust) , Stephanie Needham (Newcastle upon Tyne Hospitals NHS Foundation Trust) , Richard R. Brady (Newcastle upon Tyne Hospitals NHS Foundation Trust) , Janet E. Berrington (Royal Victoria Infirmary) , Katarina Madunic (Leiden University Medical Centre) , Manfred Wuhrer (Leiden University Medical Centre) , Peter Chater (Newcastle University) , Jeffery P. Pearson (Newcastle University) , Robert Glowacki (University of Michigan Medical School) , Eric C. Martens (University of Michigan Medical School) , Fuming Zhang (Rensselaer Polytechnic Institute) , Robert J. Linhardt (Rensselaer Polytechnic Institute) , Daniel I. R. Spencer (Ludger Ltd) , David N. Bolam (Newcastle University)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Nature Communications , VOL 11

State: Published (Approved)
Published: August 2020
Diamond Proposal Number(s): 18598

Open Access Open Access

Abstract: The thick mucus layer of the gut provides a barrier to infiltration of the underlying epithelia by both the normal microbiota and enteric pathogens. Some members of the microbiota utilise mucin glycoproteins as a nutrient source, but a detailed understanding of the mechanisms used to breakdown these complex macromolecules is lacking. Here we describe the discovery and characterisation of endo-acting enzymes from prominent mucin-degrading bacteria that target the polyLacNAc structures within oligosaccharide side chains of both animal and human mucins. These O-glycanases are part of the large and diverse glycoside hydrolase 16 (GH16) family and are often lipoproteins, indicating that they are surface located and thus likely involved in the initial step in mucin breakdown. These data provide a significant advance in our knowledge of the mechanism of mucin breakdown by the normal microbiota. Furthermore, we also demonstrate the potential use of these enzymes as tools to explore changes in O-glycan structure in a number of intestinal disease states.

Journal Keywords: Glycobiology

Diamond Keywords: Gut Microbiota; Bacteria; Enzymes

Subject Areas: Biology and Bio-materials, Medicine

Instruments: I03-Macromolecular Crystallography , I04-1-Macromolecular Crystallography (fixed wavelength) , I24-Microfocus Macromolecular Crystallography

Added On: 19/08/2020 14:05


Discipline Tags:

Health & Wellbeing Structural biology Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)