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Beamline B21: high-throughput small-angle X-ray scattering at Diamond Light Source

DOI: 10.1107/S1600577520009960 DOI Help

Authors: Nathan P. Cowieson (Diamond Light Source) , Charlotte J. C. Edwards-Gayle (Diamond Light Source of Reading) , Katsuaki Inoue (Diamond Light Source) , Nikul S. Khunti (Diamond Light Source) , James Doutch (ISIS Neutron and Muon Source) , Eugene Williams (Diamond Light Source) , Steven Daniels (Diamond Light Source) , Geoff Preece (Diamond Light Source) , Nicholas A. Krumpa (Science and Technology Facilities Council) , John P. Sutter (Diamond Light Source) , Mark D. Tully (European Synchrotron Radiation Facility (ESRF)) , Nick J. Terrill (Diamond Light Source) , Robert P. Rambo (Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal Of Synchrotron Radiation , VOL 27

State: Published (Approved)
Published: September 2020

Open Access Open Access

Abstract: B21 is a small-angle X-ray scattering (SAXS) beamline with a bending magnet source in the 3 GeV storage ring at the Diamond Light Source Ltd synchrotron in the UK. The beamline utilizes a double multi-layer monochromator and a toroidal focusing optic to deliver 2 × 1012 photons per second to a 34 × 40 µm (FWHM) focal spot at the in-vacuum Eiger 4M (Dectris) detector. A high-performance liquid chromatography system and a liquid-handling robot make it possible to load solution samples into a temperature-controlled in-vacuum sample cell with a high level of automation. Alternatively, a range of viscous or solid materials may be loaded manually using a range of custom sample cells. A default scattering vector range from 0.0026 to 0.34 Å−1 and low instrument background make B21 convenient for measuring a wide range of biological macromolecules. The beamline has run a full user programme since 2013.

Journal Keywords: SAXS; proteins; biological macromolecules; solution samples

Subject Areas: Biology and Bio-materials

Instruments: B21-High Throughput SAXS

Added On: 24/08/2020 10:36


Discipline Tags:

Life Sciences & Biotech

Technical Tags:

Scattering Small Angle X-ray Scattering (SAXS)