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Structure of a nucleotide pyrophosphatase/phosphodiesterase (NPP) from Euphorbia characias latex characterized by small-angle X-ray scattering: clues for the general organization of plant NPPs

DOI: 10.1107/S2059798320010207 DOI Help

Authors: Annalaura Sabatucci (University of Teramo) , Francesca Pintus (University of Cagliari) , Tiziana Cabras (University of Cagliari) , Federica Vincenzoni (Università Cattolica del Sacro Cuore) , Mauro Maccarrone (Campus Bio-Medico University; European Center for Brain Research (CERC), IRCCS Santa Lucia Foundation) , Rosaria Medda (University of Cagliari) , Enrico Dainese (University of Teramo)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section D Structural Biology , VOL 76 , PAGES 857 - 867

State: Published (Approved)
Published: September 2020

Abstract: Little information is available concerning the structural features of nucleotide pyrophosphatase/phosphodiesterases (NPPs) of plant origin and the crystal structures of these proteins have not yet been reported. The aim of this study was to obtain insight into these aspects by carrying out a comparative analysis of the sequences of two different fragments of an NPP from the latex of the Mediterranean shrub Euphorbia characias (ELNPP) and by studying the low-resolution structure of the purified protein in solution by means of small-angle X-ray scattering. This is the first structure of a plant NPP in solution that has been reported to date. It is shown that the ELNPP sequence is highly conserved in many other plant species. Of note, the catalytic domains of these plant NPPs have the same highly conserved PDE-domain organization as mammalian NPPs. Moreover, ELNPP is a dimer in solution and this oligomerization state is likely to be common to other plant enzymes.

Journal Keywords: Euphorbia characias; nucleotide pyrophosphatase/phosphodiesterase; SAXS; structure in solution; primary-structure comparison; catalytic domain spatial organization

Diamond Keywords: Enzymes

Subject Areas: Biology and Bio-materials

Instruments: B21-High Throughput SAXS

Added On: 03/09/2020 19:12

Discipline Tags:

Plant science Structural biology Life Sciences & Biotech

Technical Tags:

Scattering Small Angle X-ray Scattering (SAXS)