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Peptide nanoparticles for gene packaging and intracellular delivery

DOI: 10.1007/978-1-0716-0928-6 DOI Help

Authors: Maxim G. Ryadnov (National Physical Laboratory) , Paula Vila-gómez (National Physical Laboratory) , James E. Noble (National Physical Laboratory)
Co-authored by industrial partner: No

Type: Book Chapter

Book Chapter: 3

State: Published (Approved)
Published: August 2020

Abstract: Efficient gene transfer is necessary for advanced biotechnologies ranging from gene therapy to synthetic biology. Peptide nanoparticles provide suitable packaging systems promoting targeted gene expression or silencing. Though these systems have yet to match the transfection efficacy of viruses, they are typically devoid of drawbacks characteristic of virus-based vectors, including insertional mutagenesis, low packaging capacities, and strong immune responses. Given the promise nanoparticle formulations hold for gene delivery, methods of their preparation and accurate analysis of their physicochemical and biological properties become indispensable for progress toward systems that seek to outperform viral vectors. Herein, we report a comprehensive protocol for the preparation and characterization of archetypal peptide nanoparticles resulting from nonspecific and noncovalent complexation with RNA and DNA.

Journal Keywords: Nanoparticles; Gene therapy; Synthetic biology; Intracellular gene delivery; Peptide self-assembly

Subject Areas: Biology and Bio-materials


Instruments: B21-High Throughput SAXS