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Broad and strong memory CD4+ and CD8+ T cells induced by SARS-CoV-2 in UK convalescent individuals following COVID-19
DOI:
10.1038/s41590-020-0782-6
Authors:
Yanchun
Peng
(MRC Human Immunology Unit, University of Oxford; Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI))
,
Alexander J.
Mentzer
(Wellcome Centre for Human Genetics, University of Oxford; Oxford University Hospitals NHS Foundation Trust)
,
Guihai
Liu
(Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI), University of Oxford; Beijing You’an Hospital, Capital Medical University)
,
Xuan
Yao
(MRC Human Immunology Unit, University of Oxford; Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI))
,
Zixi
Yin
(MRC Human Immunology Unit, University of Oxford; Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI))
,
Danning
Dong
(MRC Human Immunology Unit, University of Oxford; Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI); Xinjiang Tumor Hospital, Xinjiang Medical University)
,
Wanwisa
Dejnirattisai
(University of Oxford)
,
Timothy
Rostron
(University of Oxford)
,
Piyada
Supasa
(University of Oxford)
,
Chang
Liu
(Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI), University of Oxford)
,
César
López-Camacho
(Wellcome Centre for Human Genetics, University of Oxfor)
,
Jose
Slon-Campos
(University of Oxford)
,
Yuguang
Zhao
(University of Oxford)
,
David I.
Stuart
(Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI); Wellcome Centre for Human Genetics, University of Oxford; Diamond Light Source)
,
Guido C.
Paesen
(Wellcome Centre for Human Genetics, University of Oxford)
,
Jonathan M.
Grimes
(Wellcome Centre for Human Genetics, University of Oxford; Diamond Light Source)
,
Alfred A.
Antson
(University of York)
,
Oliver W.
Bayfield
(University of York)
,
Dorothy E. D. P.
Hawkins
(University of York)
,
De-Sheng
Ker
(University of York)
,
Beibei
Wang
(Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI), University of Oxford)
,
Lance
Turtle
(Liverpool University Hospitals NHS Foundation Trust; University of Liverpool)
,
Krishanthi
Subramaniam
(University of Liverpool)
,
Paul
Thomson
(University of Liverpool)
,
Ping
Zhang
(University of Oxford)
,
Christina
Dold
(University of Oxford)
,
Jeremy
Ratcliff
(University of Oxford)
,
Peter
Simmonds
(University of Oxford)
,
Thushan
De Silva
(University of Sheffield)
,
Paul
Sopp
(University of Oxford)
,
Dannielle
Wellington
(Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI), University of Oxford)
,
Ushani
Rajapaksa
(Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI), University of Oxford)
,
Yi-Ling
Chen
(University of Oxford)
,
Mariolina
Salio
(University of Oxford)
,
Giorgio
Napolitani
(University of Oxford)
,
Wayne
Paes
(University of Oxford)
,
Persephone
Borrow
(University of Oxford)
,
Benedikt M.
Kessler
(Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI), University of Oxford)
,
Jeremy W.
Fry
(ProImmune)
,
Nikolai F.
Schwabe
(ProImmune)
,
Malcolm G.
Semple
(University of Liverpool; Alder Hey Children’s Hospital)
,
J. Kenneth
Baillie
(University of Edinburgh)
,
Shona C.
Moore
(University of Liverpool)
,
Peter J. M.
Openshaw
(Imperial College London)
,
M. Azim
Ansari
(University of Oxford)
,
Susanna
Dunachie
(University of Oxford; Oxford University Hospitals NHS Foundation Trust)
,
Eleanor
Barnes
(University of Oxford; Oxford University Hospitals NHS Foundation Trust; NIHR Oxford Biomedical Research Centre)
,
John
Frater
(University of Oxford; Oxford University Hospitals NHS Foundation Trust)
,
Georgina
Kerr
(University of Oxford)
,
Oliver
Gould
(University of Oxford; Oxford University Hospitals NHS Foundation Trust)
,
Teresa
Lockett
(Oxford University Hospitals NHS Foundation Trust)
,
Robert
Levin
(Worthing Hospital)
,
Yonghong
Zhang
(Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI), University of Oxford; Beijing You’an Hospital, Capital Medical University)
,
Ronghua
Jing
(Beijing You’an Hospital, Capital Medical University)
,
Ling-Pei
Ho
(Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI), University of Oxford; NIHR Oxford Biomedical Research Centre)
,
Richard J.
Cornall
(University of Oxford; Oxford University Hospitals NHS Foundation Trust)
,
Christopher P.
Conlon
(Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI), University of Oxford; Oxford University Hospitals NHS Foundation Trust)
,
Paul
Klenerman
(University of Oxford; NIHR Oxford Biomedical Research Centre)
,
Gavin R.
Screaton
(University of Oxford; Oxford University Hospitals NHS Foundation Trust; NIHR Oxford Biomedical Research Centre)
,
Juthathip
Mongkolsapaya
(Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI), University of Oxford; NIHR Oxford Biomedical Research Centre; Siriraj Hospital, Mahidol University)
,
Andrew
Mcmichael
(Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI), University of Oxford)
,
Julian C.
Knight
(Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI); Wellcome Centre for Human Genetics, University of Oxford; Oxford University Hospitals NHS Foundation Trust)
,
Graham
Ogg
(Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI), University of Oxford; Oxford University Hospitals NHS Foundation Trust; NIHR Oxford Biomedical Research Centre)
,
Tao
Dong
(Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI), University of Oxford)
Co-authored by industrial partner:
No
Type:
Journal Paper
Journal:
Nature Immunology
, VOL 7
State:
Published (Approved)
Published:
September 2020

Abstract: The development of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines and therapeutics will depend on understanding viral immunity. We studied T cell memory in 42 patients following recovery from COVID-19 (28 with mild disease and 14 with severe disease) and 16 unexposed donors, using interferon-γ-based assays with peptides spanning SARS-CoV-2 except ORF1. The breadth and magnitude of T cell responses were significantly higher in severe as compared with mild cases. Total and spike-specific T cell responses correlated with spike-specific antibody responses. We identified 41 peptides containing CD4+ and/or CD8+ epitopes, including six immunodominant regions. Six optimized CD8+ epitopes were defined, with peptide–MHC pentamer-positive cells displaying the central and effector memory phenotype. In mild cases, higher proportions of SARS-CoV-2-specific CD8+ T cells were observed. The identification of T cell responses associated with milder disease will support an understanding of protective immunity and highlights the potential of including non-spike proteins within future COVID-19 vaccine design.
Journal Keywords: Immunological memory; Viral infection
Diamond Keywords: COVID-19; Viruses
Subject Areas:
Biology and Bio-materials,
Medicine
Technical Areas:
Added On:
07/09/2020 14:21
Documents:
s41590-020-0782-6.pdf
Discipline Tags:
Vaccines
Pathogens
Infectious Diseases
Health & Wellbeing
Drug Discovery
Life Sciences & Biotech
Technical Tags: