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Infrared microspectroscopy and imaging analysis of inflammatory and non-inflammatory breast cancer cells and their GAG secretome
DOI:
10.3390/molecules25184300
Authors:
Hossam Taha
Mohamed
(Université de Reims Champagne-Ardenne; CNRS UMR 7369; Cairo University; October University for Modern Sciences and Arts)
,
Valerie
Untereiner
(Université de Reims Champagne-Ardenne)
,
Gianfelice
Cinque
(Diamond Light Source)
,
Sherif Abdelaziz
Ibrahim
(Cairo University)
,
Martin
Götte
(Münster University Hospital)
,
Nguyet Que
Nguyen
(Diamond Light Source)
,
Romain
Rivet
(Université de Reims Champagne-Ardenne; CNRS UMR 7369)
,
Ganesh D.
Sockalingum
(Université de Reims Champagne-Ardenne)
,
Stephane
Brezillon
(Université de Reims Champagne-Ardenne; CNRS UMR 7369)
Co-authored by industrial partner:
No
Type:
Journal Paper
Journal:
Molecules
, VOL 25
State:
Published (Approved)
Published:
September 2020
Diamond Proposal Number(s):
15393

Abstract: Glycosaminoglycans (GAGs)/proteoglycans (PGs) play a pivotal role in the metastasis of inflammatory breast cancer (IBC). They represent biomarkers and targets in diagnosis and treatment of different cancers including breast cancer. Thus, GAGs/PGs could represent potential prognostic/diagnostic biomarkers for IBC. In the present study, non-IBC MDA-MB-231, MCF7, SKBR3 cells and IBC SUM149 cells, as well as their GAG secretome were analyzed. The latter was measured in toto as dried drops with high-throughput (HT) Fourier Transform InfraRed (FTIR) spectroscopy and imaging. FTIR imaging was also employed to investigate single whole breast cancer cells while synchrotron-FTIR microspectroscopy was used to specifically target their cytoplasms. Data were analyzed by hierarchical cluster analysis and principal components analysis. Results obtained from HT-FTIR analysis of GAG drops showed that the inter-group variability enabled us to delineate between cell types in the GAG absorption range 1350–800 cm−1. Similar results were obtained for FTIR imaging of GAG extracts and fixed single whole cells. Synchrotron-FTIR data from cytoplasms allowed discrimination between non-IBC and IBC. Thus, by using GAG specific region, not only different breast cancer cell lines could be differentiated, but also non-IBC from IBC cells. This could be a potential diagnostic spectral marker for IBC detection useful for patient management.
Journal Keywords: inflammatory breast cancer; glycosaminoglycans; proteoglycans; secretome; infrared (micro)spectroscopy; imaging; synchrotron-FTIR
Subject Areas:
Biology and Bio-materials
Instruments:
B22-Multimode InfraRed imaging And Microspectroscopy
Documents:
molecules-25-04300.pdf