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Infrared microspectroscopy and imaging analysis of inflammatory and non-inflammatory breast cancer cells and their GAG secretome

DOI: 10.3390/molecules25184300 DOI Help

Authors: Hossam Taha Mohamed (Université de Reims Champagne-Ardenne; CNRS UMR 7369; Cairo University; October University for Modern Sciences and Arts) , Valerie Untereiner (Université de Reims Champagne-Ardenne) , Gianfelice Cinque (Diamond Light Source) , Sherif Abdelaziz Ibrahim (Cairo University) , Martin Götte (Münster University Hospital) , Nguyet Que Nguyen (Diamond Light Source) , Romain Rivet (Université de Reims Champagne-Ardenne; CNRS UMR 7369) , Ganesh D. Sockalingum (Université de Reims Champagne-Ardenne) , Stephane Brezillon (Université de Reims Champagne-Ardenne; CNRS UMR 7369)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Molecules , VOL 25

State: Published (Approved)
Published: September 2020
Diamond Proposal Number(s): 15393

Open Access Open Access

Abstract: Glycosaminoglycans (GAGs)/proteoglycans (PGs) play a pivotal role in the metastasis of inflammatory breast cancer (IBC). They represent biomarkers and targets in diagnosis and treatment of different cancers including breast cancer. Thus, GAGs/PGs could represent potential prognostic/diagnostic biomarkers for IBC. In the present study, non-IBC MDA-MB-231, MCF7, SKBR3 cells and IBC SUM149 cells, as well as their GAG secretome were analyzed. The latter was measured in toto as dried drops with high-throughput (HT) Fourier Transform InfraRed (FTIR) spectroscopy and imaging. FTIR imaging was also employed to investigate single whole breast cancer cells while synchrotron-FTIR microspectroscopy was used to specifically target their cytoplasms. Data were analyzed by hierarchical cluster analysis and principal components analysis. Results obtained from HT-FTIR analysis of GAG drops showed that the inter-group variability enabled us to delineate between cell types in the GAG absorption range 1350–800 cm−1. Similar results were obtained for FTIR imaging of GAG extracts and fixed single whole cells. Synchrotron-FTIR data from cytoplasms allowed discrimination between non-IBC and IBC. Thus, by using GAG specific region, not only different breast cancer cell lines could be differentiated, but also non-IBC from IBC cells. This could be a potential diagnostic spectral marker for IBC detection useful for patient management.

Journal Keywords: inflammatory breast cancer; glycosaminoglycans; proteoglycans; secretome; infrared (micro)spectroscopy; imaging; synchrotron-FTIR

Subject Areas: Biology and Bio-materials

Instruments: B22-Multimode InfraRed imaging And Microspectroscopy