Multiscale characterization of embryonic long bone mineralization in mice

DOI: 10.1002/advs.202002524

Authors: Isabella Silva Barreto (Lund University) , Sophie Le Cann (Lund University) , Saima Ahmed (Imperial College London) , Vivien Sotiriou (Imperial College London) , Mikael J. Turunen (University of Eastern Finland) , Ulf Johansson (MAX IV Laboratory) , Angel Rodriguez-Fernandez (MAX IV Laboratory) , Tilman A. Grünewald (European Synchrotron Radiation Facility) , Marianne Liebi (Chalmers University of Technology) , Niamh C. Nowlan (Imperial College London) , Hanna Isaksson (Lund University)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Advanced Science , VOL 143

State: Published (Approved)
Published: September 2020
Diamond Proposal Number(s): 14789 , 16557

Abstract: Long bone mineralization occurs through endochondral ossification, where a cartilage template mineralizes into bone‐like tissue with a hierarchical organization from the whole bone‐scale down to sub‐nano scale. Whereas this process has been extensively studied at the larger length scales, it remains unexplored at some of the smaller length scales. In this study, the changes in morphology, composition, and structure during embryonic mineralization of murine humeri are investigated using a range of high‐resolution synchrotron‐based imaging techniques at several length scales. With micro‐ and nanometer spatial resolution, the deposition of elements and the shaping of mineral platelets are followed. Rapid mineralization of the humeri occurs over approximately four days, where mineral to matrix ratio and calcium content in the most mineralized zone reach adult values shortly before birth. Interestingly, zinc is consistently found to be localized at the sites of ongoing new mineralization. The mineral platelets in the most recently mineralized regions are thicker, longer, narrower, and less aligned compared to those further into the mineralized region. In summary, this study demonstrates a specific spatial distribution of zinc, with highest concentration where new mineral is being deposited and that the newly formed mineral platelets undergo slight reshaping and reorganization during embryonic development.

Journal Keywords: bone development; Fourier transform infra‐red microspectroscopy; small‐ and wide‐angle X‐ray scattering; X‐ray fluorescence spectroscopy; X‐ray tomography

Diamond Keywords: Bone

Subject Areas: Biology and Bio-materials


Instruments: I13-2-Diamond Manchester Imaging

Other Facilities: ID13 beamline at ESRF NanoMax beamline at Max-IV

Added On: 01/10/2020 11:40

Documents:
advs.202002524.pdf

Discipline Tags:

Health & Wellbeing Life Sciences & Biotech

Technical Tags:

Imaging Tomography