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Establishment of long-term ostracod epidermal culture

DOI: 10.1007/s11626-020-00508-8 DOI Help

Authors: Siân R. Morgan (Cardiff University) , Laura Paletto (Cardiff University) , Benjamin Rumney (Cardiff University) , Farhana T. Malik (Lifescaped; University of Oxford) , Nick White (Cardiff University) , Philip N. Lewis (Cardiff University) , Andrew R. Parker (Lifescaped; University of Oxford) , Simon Holden (Defence Science and Technology Laboratory (DSTL)) , Keith M. Meek (Cardiff University) , Julie Albon (Cardiff University)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: In Vitro Cellular & Developmental Biology - Animal , VOL 16

State: Published (Approved)
Published: October 2020
Diamond Proposal Number(s): 17839

Open Access Open Access

Abstract: Primary crustacean cell culture was introduced in the 1960s, but to date limited cell lines have been established. Skogsbergia lerneri is a myodocopid ostracod, which has a body enclosed within a thin, durable, transparent bivalved carapace, through which the eye can see. The epidermal layer lines the inner surface of the carapace and is responsible for carapace synthesis. The purpose of the present study was to develop an in vitro epidermal tissue and cell culture method for S. lerneri. First, an optimal environment for the viability of this epidermal tissue was ascertained, while maintaining its cell proliferative capacity. Next, a microdissection technique to remove the epidermal layer for explant culture was established and finally, a cell dissociation method for epidermal cell culture was determined. Maintenance of sterility, cell viability and proliferation were key throughout these processes. This novel approach for viable S. lerneri epidermal tissue and cell culture augments our understanding of crustacean cell biology and the complex biosynthesis of the ostracod carapace. In addition, these techniques have great potential in the fields of biomaterial manufacture, the military and fisheries, for example, in vitro toxicity testing.

Journal Keywords: Crustacean; Ostracod; Carapace; Epidermal; Culture

Subject Areas: Biology and Bio-materials

Instruments: I13-2-Diamond Manchester Imaging


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