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Three-dimensional cotton-wool-like polyhydroxybutyrate/siloxane-doped vaterite composite fibrous scaffolds: Effect of imogolite-coating on physicochemical and cell adhesion properties

DOI: 10.3389/fmats.2020.00033 DOI Help

Authors: Akiko Obata (Nagoya Institute of Technology) , Kazuma Mori (Nagoya Institute of Technology) , Keiichi Inukai (National Institute of Advanced Industrial Science and Technology (AIST), Japan) , Katsuya Kato (National Institute of Advanced Industrial Science and Technology (AIST), Japan) , Gowsihan Poologasundarampillai (University of Birmingham) , Toshihiro Kasuga (Nagoya Institute of Technology)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Frontiers In Materials , VOL 7

State: Published (Approved)
Published: February 2020
Diamond Proposal Number(s): 11079

Open Access Open Access

Abstract: Poly(3-hydroxybutyrate-co-4-hydroxybutyrate), P(3HB-4HB), and siloxane-doped vaterite (SiV) composite fibrous scaffolds with 3D cotton-wool-like structure were developed using an electrospinning system for use in bone tissue regeneration. Scaffolds exhibited a significantly larger fiber-fiber separation distribution than non-woven fiber mats as observed with micro-computed tomographic studies. Coating the hydrophobic P(3HB-4HB)/SiV fibers with imogolite nanotubes (INT), aluminum silicate nanotubes, made the 3D construct hydrophilic and improved water penetration into the 3D structure (~2 s). Coating efficacy was confirmed by the detection of aluminum on the surface of fibers using scanning electron microscopy (SEM) energy dispersive spectroscopy (EDS). Dissolution experiments showed increased release of silicate ions in cell culture medium which can improve migration and mineralization of osteogenic cells inside of the 3D structure. The coating also contributed to an enhanced adhesion and migration of osteoblast-like cells (SaOS-2) within the 3D construct. The differentiation and mineralization of the cells were not affected by the coating. The coating for such cotton-wool-like structured scaffolds was effective for an enhancement of cell functions on early stages of culture. Thus, the developed materials with 3D structure, flexibility, silicate-ion release ability, and cell compatibility are expected to be good candidate materials for bone tissue regeneration.

Journal Keywords: cotton-wool like structure; polyhydroxyalkanoates; imogolite; electrospinning; cell adhesion; 3D ╬╝CT quantification

Diamond Keywords: Bone

Subject Areas: Materials, Chemistry


Instruments: I13-2-Diamond Manchester Imaging

Added On: 15/10/2020 10:43

Documents:
fmats-07-00033.pdf

Discipline Tags:

Biomaterials Chemistry Materials Science Organic Chemistry Polymer Science

Technical Tags:

Imaging Tomography