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Screening and production of recombinant human proteins: protein production in insect cells

DOI: 10.1007/978-1-0716-0892-0_5 DOI Help

Authors: Pravin Mahajan (Astex Pharmaceuticals) , Claire Strain-damerell (Diamond Light Source) , Shubhashish Mukhopadhyay (Structural Genomics Consortium, University of Oxford) , Alejandra Fernandez-cid (Structural Genomics Consortium, University of Oxford) , Opher Gileadi (Structural Genomics Consortium, University of Oxford) , Nicola A. Burgess-brown (Structural Genomics Consortium, University of Oxford)
Co-authored by industrial partner: Yes

Type: Book Chapter

Book Chapter: 5

State: Published (Approved)
Published: October 2020

Abstract: This chapter describes the step-by-step methods employed by the Structural Genomics Consortium (SGC) for screening and producing proteins in the baculovirus expression vector system (BEVS). This eukaryotic expression system was selected and a screening process established in 2007 as a measure to tackle the more challenging kinase, RNA–DNA processing, and integral membrane protein families on our target list. Here, we discuss our platform for identifying soluble proteins from 3 mL of insect cell culture and describe the procedures involved in producing protein from liter-scale cultures.

Journal Keywords: Insect cells; Baculovirus; BEVS; Expression; Recombinant; Protein; Purification; IMAC; SEC chromatography; Gel filtration

Subject Areas: Biology and Bio-materials

Technical Areas: