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Diffraction data from aerosolized Coliphage PR772 virus particles imaged with the Linac Coherent Light Source

DOI: 10.1038/s41597-020-00745-2 DOI Help

Authors: Haoyuan Li (SLAC National Accelerator Laboratory; Stanford University) , Reza Nazari (Arizona State University) , Brian Abbey (La Trobe University) , Roberto Alvarez (Arizona State University) , Andrew Aquila (SLAC National Accelerator Laboratory) , Kartik Ayyer (Max Planck Institute for the Structure and Dynamics of Matter; DESY) , Anton Barty (DESY) , Peter Berntsen (La Trobe University) , Johan Bielecki (European XFEL; Uppsala University) , Alberto Pietrini (Uppsala University) , Maximilian Bucher (SLAC National Accelerator Laboratory) , Gabriella Carini (Brookhaven National Laboratory) , Henry N. Chapman (DESY; Centre for Ultrafast Imaging) , Alice Contreras (Arizona State University) , Benedikt J. Daurer (Uppsala University; Diamond Light Source) , Hasan Demirci (Stanford PULSE Institute; Koc University) , Leonie Flűckiger (La Trobe University) , Matthias Frank (Lawrence Livermore National Laboratory) , Janos Hajdu (Uppsala University; The European Extreme Light Infrastructure, Institute of Physics, Academy of Sciences of the Czech Republic) , Max F. Hantke (Uppsala University) , Brenda G. Hogue (Arizona State University) , Ahmad Hosseinizadeh (University of Wisconsin Milwaukee) , Mark S. Hunter (SLAC National Accelerator Laboratory) , H. Olof Jönsson (AlbaNova University; KTH Royal Institute of Technology) , Richard A. Kirian (Arizona State University) , Ruslan P. Kurta (European XFEL) , Duane Loh (National University of Singapore) , Filipe R. N. C. Maia (Uppsala University) , Adrian P. Mancuso (European XFEL; La Trobe University) , Andrew J. Morgan (University of Melbourne) , Matthew Mcfadden (Arizona State University) , Kerstin Muehlig (Uppsala University) , Anna Munke (Uppsala University) , Hemanth Kumar Narayana Reddy (Uppsala University) , Carl Nettelblad (Uppsala University) , Abbas Ourmazd (University of Wisconsin Milwaukee) , Max Rose (DESY) , Peter Schwander (University of Wisconsin Milwaukee) , M. Marvin Seibert (Uppsala University) , Jonas A. Sellberg (AlbaNova University; KTH Royal Institute of Technology) , Raymond G. Sierra (SLAC National Accelerator Laboratory) , Zhibin Sun (SLAC National Accelerator Laboratory; Paul Scherrer Institute) , Martin Svenda (KTH Royal Institute of Technology,) , Ivan A. Vartanyants (DESY; NRNU MEPhI) , Peter Walter (SLAC National Accelerator Laboratory) , Daniel Westphal (Uppsala University) , Garth Williams (Brookhaven National Laboratory) , P. Lourdu Xavier (SLAC National Accelerator Laboratory; Max Planck Institute for the Structure and Dynamics of Matter; DESY) , Chun Hong Yoon (SLAC National Accelerator Laboratory) , Sahba Zaare (Arizona State University)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Scientific Data , VOL 7

State: Published (Approved)
Published: November 2020

Open Access Open Access

Abstract: Single Particle Imaging (SPI) with intense coherent X-ray pulses from X-ray free-electron lasers (XFELs) has the potential to produce molecular structures without the need for crystallization or freezing. Here we present a dataset of 285,944 diffraction patterns from aerosolized Coliphage PR772 virus particles injected into the femtosecond X-ray pulses of the Linac Coherent Light Source (LCLS). Additional exposures with background information are also deposited. The diffraction data were collected at the Atomic, Molecular and Optical Science Instrument (AMO) of the LCLS in 4 experimental beam times during a period of four years. The photon energy was either 1.2 or 1.7 keV and the pulse energy was between 2 and 4 mJ in a focal spot of about 1.3 μm x 1.7 μm full width at half maximum (FWHM). The X-ray laser pulses captured the particles in random orientations. The data offer insight into aerosolised virus particles in the gas phase, contain information relevant to improving experimental parameters, and provide a basis for developing algorithms for image analysis and reconstruction.

Subject Areas: Biology and Bio-materials

Facility: Linac Coherent Light Source

Documents:
s41597-020-00745-2.pdf