Evidence of escape of SARS-CoV-2 variant B.1.351 from natural and vaccine induced sera

DOI: 10.1016/j.cell.2021.02.037

Authors: Daming Zhou (The Wellcome Centre for Human Genetics, University of Oxford) , Wanwisa Dejnirattisai (The Wellcome Centre for Human Genetics, University of Oxford) , Piyada Supasa (The Wellcome Centre for Human Genetics, University of Oxford) , Chang Liu (The Wellcome Centre for Human Genetics, University of Oxford; Chinese Academy of Medical Science (CAMS) Oxford Institute (COI), University of Oxford) , Alexander J. Mentzer (The Wellcome Centre for Human Genetics, University of Oxford; Oxford University Hospitals NHS Foundation Trust) , Helen M. Ginn (The Wellcome Centre for Human Genetics, University of Oxford) , Yuguang Zhao (The Wellcome Centre for Human Genetics, University of Oxford) , Helen M. E. Duyvesteyn (The Wellcome Centre for Human Genetics, University of Oxford) , Aekkachai Tuekprakhon (The Wellcome Centre for Human Genetics, University of Oxford) , Rungtiwa Nutalai (The Wellcome Centre for Human Genetics, University of Oxford) , Beibei Wang (The Wellcome Centre for Human Genetics, University of Oxford) , Guido C. Paesen (The Wellcome Centre for Human Genetics, University of Oxford) , Cesar Lopez-Camacho (The Wellcome Centre for Human Genetics, University of Oxford) , Jose Slon-Campos (The Wellcome Centre for Human Genetics, University of Oxford) , Bassam Hallis (Public Health England (PHE)) , Naomi Coombes (Public Health England (PHE)) , Kevin Bewley (Public Health England (PHE)) , Sue Charlton (Public Health England (PHE)) , Thomas S. Walter (The Wellcome Centre for Human Genetics, University of Oxford) , Donal Skelly (Oxford University Hospitals NHS Foundation Trust; University of Oxford) , Sheila F. Lumley (University of Oxford) , Christina Dold (NIHR Oxford Biomedical Research Centre; University of Oxford) , Robert Levin (Worthing Hospital) , Tao Dong (Chinese Academy of Medical Science (CAMS) Oxford Institute (COI), University of Oxford) , Andrew J. Pollard (NIHR Oxford Biomedical Research Centre; University of Oxford) , Julian C. Knight (The Wellcome Centre for Human Genetics, University of Oxford; Oxford University Hospitals NHS Foundation Trust) , Derrick Crook (University of Oxford) , Teresa Lambe (Jenner Institute, University of Oxford) , Elizabeth Clutterbuck (NIHR Oxford Biomedical Research Centre; University of Oxford) , Sagida Bibi (NIHR Oxford Biomedical Research Centre; University of Oxford) , Amy Flaxman (Jenner Institute, University of Oxford) , Mustapha Bittaye (Jenner Institute, University of Oxford) , Sandra Belij-Rammerstorfer (Jenner Institute, University of Oxford) , Sarah Gilbert (Jenner Institute, University of Oxford) , William James (University of Oxford) , Miles W. Carroll (The Wellcome Centre for Human Genetics, University of Oxford; Public Health England (PHE)) , Paul Klenerman (Oxford University Hospitals NHS Foundation Trust; NIHR Oxford Biomedical Research Centre; University of Oxford) , Eleanor Barnes (Oxford University Hospitals NHS Foundation Trust; NIHR Oxford Biomedical Research Centre; University of Oxford) , Susanna J. Dunachie (Oxford University Hospitals NHS Foundation Trust; University of Oxford; Mahidol-Oxford Tropical Medicine Research Unit, Bangkok) , Elizabeth E. Fry (The Wellcome Centre for Human Genetics, University of Oxford) , Juthathip Mongkolspaya (The Wellcome Centre for Human Genetics, University of Oxford; Chinese Academy of Medical Science (CAMS) Oxford Institute (COI), University of Oxford; Mahidol University) , Jingshan Ren (The Wellcome Centre for Human Genetics, University of Oxford) , David I. Stuart (The Wellcome Centre for Human Genetics, University of Oxford; Chinese Academy of Medical Science (CAMS) Oxford Institute (COI), University of Oxford; Diamond Light Source; Instruct-ERIC) , Gavin R. Screaton (The Wellcome Centre for Human Genetics, University of Oxford; Chinese Academy of Medical Science (CAMS) Oxford Institute (COI), University of Oxford)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Cell

State: Published (Approved)
Published: February 2021
Diamond Proposal Number(s): 27009

Abstract: The race to produce vaccines against SARS-CoV-2 began when the first sequence was published, and this forms the basis for vaccines currently deployed globally. Independent lineages of SARS-CoV-2 have recently been reported: UK–B.1.1.7, South Africa–B.1.351 and Brazil–P.1. These variants have multiple changes in the immunodominant spike protein which facilitates viral cell entry via the Angiotensin converting enzyme-2 (ACE2) receptor. Mutations in the receptor recognition site on the spike are of great concern for their potential for immune escape. Here we describe a structure-function analysis of B.1.351 using a large cohort of convalescent and vaccinee serum samples. The receptor binding domain mutations provide tighter ACE2 binding and widespread escape from monoclonal antibody neutralization largely driven by E484K although K417N and N501Y act together against some important antibody classes. In a number of cases it would appear that convalescent and some vaccine serum offers limited protection against this variant.

Diamond Keywords: COVID-19; Viruses

Subject Areas: Biology and Bio-materials, Medicine


Instruments: I03-Macromolecular Crystallography

Added On: 24/02/2021 08:58

Discipline Tags:

Vaccines Infectious Diseases Health & Wellbeing Structural biology Drug Discovery Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)