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Tomographic X-ray scattering based on invariant reconstruction: analysis of the 3D nanostructure of bovine bone

DOI: 10.1107/S1600576721000881 DOI Help

Authors: Paolino De Falco (Max Planck Institute of Colloids and Interfaces) , Richard Weinkamer (Max Planck Institute of Colloids and Interfaces) , Wolfgang Wagermaier (Max Planck Institute of Colloids and Interfaces) , Chenghao Li (Max Planck Institute of Colloids and Interfaces) , Tim Snow (Diamond Light Source) , Nicholas J. Terrill (Diamond Light Source) , Himadri Gupta (Queen Mary, University of London) , Pawan Goyal (Max Planck Institute for Dynamics of Complex Technical Systems) , Martin Stoll (Max Planck Institute for Dynamics of Complex Technical Systems; TU Chemnitz) , Peter Benner (Max Planck Institute for Dynamics of Complex Technical Systems) , Peter Fratzl (Max Planck Institute of Colloids and Interfaces)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal Of Applied Crystallography , VOL 54

State: Published (Approved)
Published: April 2021
Diamond Proposal Number(s): 18524

Open Access Open Access

Abstract: Small-angle X-ray scattering (SAXS) is an effective characterization technique for multi-phase nanocomposites. The structural complexity and heterogeneity of biological materials require the development of new techniques for the 3D characterization of their hierarchical structures. Emerging SAXS tomographic methods allow reconstruction of the 3D scattering pattern in each voxel but are costly in terms of synchrotron measurement time and computer time. To address this problem, an approach has been developed based on the reconstruction of SAXS invariants to allow for fast 3D characterization of nanostructured inhomogeneous materials. SAXS invariants are scalars replacing the 3D scattering patterns in each voxel, thus simplifying the 6D reconstruction problem to several 3D ones. Standard procedures for tomographic reconstruction can be directly adapted for this problem. The procedure is demonstrated by determining the distribution of the nanometric bone mineral particle thickness (T parameter) throughout a macroscopic 3D volume of bovine cortical bone. The T parameter maps display spatial patterns of particle thickness in fibrolamellar bone units. Spatial correlation between the mineral nano­structure and microscopic features reveals that the mineral particles are particularly thin in the vicinity of vascular channels.

Journal Keywords: small-angle X-ray scattering; SAXS; tomography; bovine bone; fibrolamellar unit; T parameter; scattering tomography; fibrolamellar bone

Diamond Keywords: Bone

Subject Areas: Biology and Bio-materials, Technique Development

Instruments: I22-Small angle scattering & Diffraction

Other Facilities: µSpot beamline at BESSY II


Discipline Tags:

Life Sciences & Biotech Technique Development - Life Sciences & Biotech

Technical Tags:

Scattering Small Angle X-ray Scattering (SAXS)