Publication
Article Metrics
Citations
Online attention
Tomographic X-ray scattering based on invariant reconstruction: analysis of the 3D nanostructure of bovine bone
DOI:
10.1107/S1600576721000881
Authors:
Paolino
De Falco
(Max Planck Institute of Colloids and Interfaces)
,
Richard
Weinkamer
(Max Planck Institute of Colloids and Interfaces)
,
Wolfgang
Wagermaier
(Max Planck Institute of Colloids and Interfaces)
,
Chenghao
Li
(Max Planck Institute of Colloids and Interfaces)
,
Tim
Snow
(Diamond Light Source)
,
Nicholas J.
Terrill
(Diamond Light Source)
,
Himadri
Gupta
(Queen Mary, University of London)
,
Pawan
Goyal
(Max Planck Institute for Dynamics of Complex Technical Systems)
,
Martin
Stoll
(Max Planck Institute for Dynamics of Complex Technical Systems; TU Chemnitz)
,
Peter
Benner
(Max Planck Institute for Dynamics of Complex Technical Systems)
,
Peter
Fratzl
(Max Planck Institute of Colloids and Interfaces)
Co-authored by industrial partner:
No
Type:
Journal Paper
Journal:
Journal Of Applied Crystallography
, VOL 54
State:
Published (Approved)
Published:
April 2021
Diamond Proposal Number(s):
18524

Abstract: Small-angle X-ray scattering (SAXS) is an effective characterization technique for multi-phase nanocomposites. The structural complexity and heterogeneity of biological materials require the development of new techniques for the 3D characterization of their hierarchical structures. Emerging SAXS tomographic methods allow reconstruction of the 3D scattering pattern in each voxel but are costly in terms of synchrotron measurement time and computer time. To address this problem, an approach has been developed based on the reconstruction of SAXS invariants to allow for fast 3D characterization of nanostructured inhomogeneous materials. SAXS invariants are scalars replacing the 3D scattering patterns in each voxel, thus simplifying the 6D reconstruction problem to several 3D ones. Standard procedures for tomographic reconstruction can be directly adapted for this problem. The procedure is demonstrated by determining the distribution of the nanometric bone mineral particle thickness (T parameter) throughout a macroscopic 3D volume of bovine cortical bone. The T parameter maps display spatial patterns of particle thickness in fibrolamellar bone units. Spatial correlation between the mineral nanostructure and microscopic features reveals that the mineral particles are particularly thin in the vicinity of vascular channels.
Journal Keywords: small-angle X-ray scattering; SAXS; tomography; bovine bone; fibrolamellar unit; T parameter; scattering tomography; fibrolamellar bone
Diamond Keywords: Bone
Subject Areas:
Biology and Bio-materials,
Technique Development
Instruments:
I22-Small angle scattering & Diffraction
Other Facilities: µSpot beamline at BESSY II
Added On:
09/03/2021 09:21
Documents:
vg5128.pdf
Discipline Tags:
Technique Development - Life Sciences & Biotech
Life Sciences & Biotech
Technical Tags:
Scattering
Small Angle X-ray Scattering (SAXS)