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Antibody evasion by the P.1 strain of SARS-CoV-2
DOI:
10.1016/j.cell.2021.03.055
Authors:
Wanwisa
Dejnirattisai
(Wellcome Centre for Human Genetics, University of Oxford)
,
Daming
Zhou
(The Wellcome Centre for Human Genetics, University of Oxford)
,
Piyada
Supasa
(Wellcome Centre for Human Genetics, University of Oxford)
,
Chang
Liu
(Wellcome Centre for Human Genetics, University of Oxford; Chinese Academy of Medical Science (CAMS) Oxford Institute (COI))
,
Alexander J.
Mentzer
(Wellcome Centre for Human Genetics, University of Oxford; Oxford University Hospitals NHS Foundation Trust)
,
Helen M.
Ginn
(University of Oxford)
,
Yuguang
Zhao
(Wellcome Centre for Human Genetics, University of Oxford)
,
Helen M. E.
Duyvesteyn
(Wellcome Centre for Human Genetics, University of Oxford)
,
Aekkachai
Tuekprakhon
(Wellcome Centre for Human Genetics, University of Oxford)
,
Rungtiwa
Nutalai
(Wellcome Centre for Human Genetics, University of Oxford)
,
Beibei
Wang
(Wellcome Centre for Human Genetics, University of Oxford)
,
Guido
Paesen
(Wellcome Centre for Human Genetics, University of Oxford)
,
César
López-Camacho
(Wellcome Centre for Human Genetics, University of Oxford)
,
Jose
Slon-Campos
(Wellcome Centre for Human Genetics, University of Oxford)
,
Thomas S.
Walter
(Wellcome Centre for Human Genetics, University of Oxford)
,
Donal
Skelly
(Oxford University Hospitals NHS Foundation Trust; University of Oxford)
,
Sue Ann
Costa Clemens
(University of Siena; University of Oxford)
,
Felipe Gomes
Naveca
(Instituto Leônidas e Maria Deane)
,
Valdinete
Nascimento
(Instituto Leônidas e Maria Deane)
,
Fernanda
Nascimento
(Instituto Leônidas e Maria Deane)
,
Cristiano
Fernandes Da Costa
(Fundação de Vigilância em Saúde do Amazonas)
,
Paola C.
Resende
(Laboratorio de vírus respiratórios- IOC/FIOCRUZ)
,
Alex
Pauvolid-Correa
(Laboratorio de vírus respiratórios- IOC/FIOCRUZ)
,
Marilda M.
Siqueira
(Laboratorio de vírus respiratórios- IOC/FIOCRUZ)
,
Christina
Dold
(NIHR Oxford Biomedical Research Centre; University of Oxford)
,
Robert
Levin
(Worthing Hospital)
,
Tao
Dong
(Chinese Academy of Medical Science (CAMS) Oxford Institute (COI), University of Oxford; MRC Weatherall Institute of Molecular Medicine)
,
Andrew J.
Pollard
(NIHR Oxford Biomedical Research Centre; University of Oxford)
,
Julian C.
Knight
(Wellcome Centre for Human Genetics, University of Oxford; Oxford University Hospitals NHS Foundation Trust)
,
Derrick
Crook
(University of Oxford)
,
Teresa
Lambe
(Jenner Institute, University of Oxford)
,
Elizabeth
Clutterbuck
(NIHR Oxford Biomedical Research Centre; University of Oxford)
,
Sagida
Bibi
(NIHR Oxford Biomedical Research Centre; University of Oxford)
,
Amy
Flaxman
(Jenner Institute, University of Oxford)
,
Mustapha
Bittaye
(Jenner Institute, University of Oxford)
,
Sandra
Belij-Rammerstorfer
(Jenner Institute, University of Oxford)
,
Sarah
Gilbert
(Jenner Institute, University of Oxford)
,
Miles W.
Carroll
(Wellcome Centre for Human Genetics, University of Oxford; Public Health England (PHE))
,
Paul
Klenerman
(Oxford University Hospitals NHS Foundation Trust; NIHR Oxford Biomedical Research Centre; University of Oxford)
,
Eleanor
Barnes
(Oxford University Hospitals NHS Foundation Trust; NIHR Oxford Biomedical Research Centre; University of Oxford)
,
Susanna J.
Dunachie
(Oxford University Hospitals NHS Foundation Trust; Mahidol-Oxford Tropical Medicine Research Unit, Thailand; University of Oxford)
,
Neil G.
Paterson
(Diamond Light Source)
,
Mark A.
Williams
(Diamond Light Source)
,
David R.
Hall
(Diamond Light Source)
,
Ruben J. G.
Hulswit
(Wellcome Centre for Human Genetics, University of Oxford)
,
Thomas A.
Bowden
(Wellcome Centre for Human Genetics, University of Oxford)
,
Elizabeth E.
Fry
(Wellcome Centre for Human Genetics, University of Oxford)
,
Juthathip
Mongkolsapaya
(Wellcome Centre for Human Genetics, University of Oxford; Chinese Academy of Medical Science (CAMS) Oxford Institute (COI); Mahidol University)
,
Jingshan
Ren
(Wellcome Centre for Human Genetics, University of Oxford)
,
David I.
Stuart
(Wellcome Centre for Human Genetics, University of Oxford; Chinese Academy of Medical Science (CAMS) Oxford Institute (COI); Diamond Light Source; Instruct-ERIC)
,
Gavin R.
Screaton
(Wellcome Centre for Human Genetics, University of Oxford; Chinese Academy of Medical Science (CAMS) Oxford Institute (COI))
Co-authored by industrial partner:
No
Type:
Journal Paper
Journal:
Cell
State:
Published (Approved)
Published:
March 2021
Diamond Proposal Number(s):
27009

Abstract: Terminating the SARS-CoV-2 pandemic relies upon pan-global vaccination. Current vaccines elicit neutralizing antibody responses to the virus spike derived from early isolates. However, new strains have emerged with multiple mutations: P.1 from Brazil, B.1.351 from South Africa and B.1.1.7 from the UK (12, 10 and 9 changes in the spike respectively). All have mutations in the ACE2 binding site with P.1 and B.1.351 having a virtually identical triplet: E484K, K417N/T and N501Y, which we show confer similar increased affinity for ACE2. We show that, surprisingly, P.1 is significantly less resistant to naturally acquired or vaccine induced antibody responses than B.1.351 suggesting that changes outside the RBD impact neutralisation. Monoclonal antibody 222 neutralises all three variants despite interacting with two of the ACE2 binding site mutations, we explain this through structural analysis and use the 222 light chain to largely restore neutralization potency to a major class of public antibodies.
Diamond Keywords: COVID-19; Viruses
Subject Areas:
Biology and Bio-materials,
Medicine
Instruments:
I03-Macromolecular Crystallography
Added On:
31/03/2021 08:47
Discipline Tags:
Vaccines
Pathogens
Infectious Diseases
Health & Wellbeing
Structural biology
Life Sciences & Biotech
Technical Tags:
Diffraction
Macromolecular Crystallography (MX)