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X-ray ptychography imaging of human chromosomes after low-dose irradiation

DOI: 10.1007/s10577-021-09660-7 DOI Help

Authors: Archana Bhartiya (University College London; Research Complex at Harwell) , Darren Batey (Diamond Light Source) , Silvia Cipiccia (Diamond Light Source) , Xiaowen Shi (Diamond Light Source; New Mexico State University) , Christoph Rau (Diamond Light Source) , Stanley Botchway (Research Complex at Harwell) , Mohammed Yusuf (University College London; Research Complex at Harwell; Aga Khan University) , Ian K. Robinson (Research Complex at Harwell; Brookhaven National Lab; University College London)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Chromosome Research , VOL 55

State: Published (Approved)
Published: March 2021

Open Access Open Access

Abstract: Studies of the structural and functional role of chromosomes in cytogenetics have spanned more than 10 decades. In this work, we take advantage of the coherent X-rays available at the latest synchrotron sources to extract the individual masses of all 46 chromosomes of metaphase human B and T cells using hard X-ray ptychography. We have produced ‘X-ray karyotypes’ of both heavy metal–stained and unstained spreads to determine the gain or loss of genetic material upon low-level X-ray irradiation doses due to radiation damage. The experiments were performed at the I-13 beamline, Diamond Light Source, Didcot, UK, using the phase-sensitive X-ray ptychography method.

Journal Keywords: X-ray imaging; X-ray microscopy; Karyotype; Irradiation; Chromosome structure; Mass determination

Subject Areas: Biology and Bio-materials


Instruments: I13-1-Coherence

Discipline Tags:

Life Sciences & Biotech Health & Wellbeing

Technical Tags:

Imaging Coherent Diffraction Imaging (CDI) Ptychography