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Alternative architecture of the E. coli chemosensory array

DOI: 10.3390/biom11040495 DOI Help

Authors: Alister Burt (Université Grenoble Alpes, CEA, CNRS, IBS) , C. Keith Cassidy (University of Oxford) , Phillip J. Stansfeld (University of Warwick) , Irina Gutsche (Université Grenoble Alpes, CEA, CNRS, IBS)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Biomolecules , VOL 11

State: Published (Approved)
Published: March 2021

Open Access Open Access

Abstract: Chemotactic responses in motile bacteria are the result of sophisticated signal transduction by large, highly organized arrays of sensory proteins. Despite tremendous progress in the understanding of chemosensory array structure and function, a structural basis for the heightened sensitivity of networked chemoreceptors is not yet complete. Here, we present cryo-electron tomography visualisations of native-state chemosensory arrays in E. coli minicells. Strikingly, these arrays appear to exhibit a p2-symmetric array architecture that differs markedly from the p6-symmetric architecture previously described in E. coli. Based on this data, we propose molecular models of this alternative architecture and the canonical p6-symmetric assembly. We evaluate our observations and each model in the context of previously published data, assessing the functional implications of an alternative architecture and effects for future studies.

Journal Keywords: chemosensory array; chemotaxis; E. coli; cryo-electron tomography; molecular modelling

Diamond Keywords: Bacteria

Subject Areas: Biology and Bio-materials

Diamond Offline Facilities: Electron Bio-Imaging Centre (eBIC)
Instruments: Krios I-Titan Krios I at Diamond

Documents:
biomolecules-11-00495-v4.pdf

Discipline Tags:

Life Sciences & Biotech Health & Wellbeing Pathogens

Technical Tags:

Imaging Tomography Cryo Electron Tomography (Cryo ET)