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Data-deposition protocols for correlative soft X-ray tomography and super-resolution structured illumination microscopy applications

DOI: 10.1016/j.xpro.2020.100253 DOI Help

Authors: Andrii Iudin (European Bioinformatics Institute) , Ugis Sarkans (European Bioinformatics Institute) , Mohamed A. Koronfel (Diamond Light Source) , Tom Fish (Diamond Light Source) , Ian Dobbie (University of Oxford) , Maria Harkiolaki (Diamond Light Source) , Ardan Patwardhan (European Bioinformatics Institute) , Gerard J. Kleywegt (European Bioinformatics Institute)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Star Protocols , VOL 2

State: Published (Approved)
Published: March 2021
Diamond Proposal Number(s): 21046 , 18314

Open Access Open Access

Abstract: This protocol illustrates the steps necessary to deposit correlated 3D cryo-imaging data from cryo-structured illumination microscopy and cryo-soft X-ray tomography with the BioStudies and EMPIAR deposition databases of the European Bioinformatics Institute. There is currently a real need for a robust method of data deposition to ensure unhindered access to and independent validation of correlative light and X-ray microscopy data to allow use in further comparative studies, educational activities, and data mining.

Journal Keywords: Bioinformatics; Cell Biology; Microscopy; Structural Biology

Subject Areas: Information and Communication Technology


Instruments: B24-Cryo Soft X-ray Tomography

Added On: 15/04/2021 10:47

Documents:
1-s2.0-S2666166720302409-main.pdf

Discipline Tags:

Data management / presentation Information & Communication Technologies Life Sciences & Biotech

Technical Tags:

Imaging Tomography Cryo-soft X-ray Tomography (Cryo-SXT)