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Protocol for image registration of correlative soft X-ray tomography and super-resolution structured illumination microscopy images

DOI: 10.1016/j.xpro.2021.100529 DOI Help

Authors: Nina Vyas (Diamond Light Source) , Stephan Kunne (Université de Nantes, CHU Nantes, CNRS UMR 6291) , Thomas M. Fish (Diamond Light Source) , Ian M. Dobbie (University of Oxford) , Maria Harkiolaki (Diamond Light Source) , Perrine Paul-Gilloteaux (Université de Nantes, CHU Nantes, CNRS UMR 6291; Université de Nantes, CHU Nantes, Inserm, CNRS, SFR Santé)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Star Protocols , VOL 2

State: Published (Approved)
Published: June 2021
Diamond Proposal Number(s): 21046 , 18314

Open Access Open Access

Abstract: Correlation of 3D images acquired on different microscopes can be a daunting prospect even for experienced users. This protocol describes steps for registration of images from soft X-ray absorption contrast imaging and super-resolution fluorescence imaging of hydrated biological materials at cryogenic temperatures. Although it is developed for data generated at synchrotron beamlines that offer the above combination of microscopies, it is applicable to all analogous imaging systems where the same area of a sample is examined using successive non-destructive imaging techniques.

Journal Keywords: Microscopy; Structural Biology

Subject Areas: Biology and Bio-materials

Instruments: B24-Cryo Soft X-ray Tomography

Added On: 11/05/2021 08:20


Discipline Tags:

Structural biology Life Sciences & Biotech

Technical Tags:

Imaging Tomography Cryo-soft X-ray Tomography (Cryo-SXT)