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Thickness determination of the tidemark of human articular cartilage using high-resolution micro-XRF imaging of zinc and lead

DOI: 10.1016/j.ocarto.2021.100182 DOI Help

Authors: M. Rauwolf (Atominstitut, TU Wien) , A. Turyanskaya (Atominstitut, TU Wien) , P. Wobrauschek (Atominstitut, TU Wien) , K. Sawhney (Diamond Light Source) , A. Roschger (Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of OEGK and AUVA Trauma Centre Meidling) , P. Roschger (Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of OEGK and AUVA Trauma Centre Meidling) , C. Streli (Atominstitut, TU Wien) , J.g. Hofstaetter (Orthopaedic Hospital Vienna-Speising; Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of OEGK and AUVA Trauma Centre Meidling)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Osteoarthritis And Cartilage Open , VOL 18

State: Published (Approved)
Published: May 2021
Diamond Proposal Number(s): 15267

Open Access Open Access

Abstract: Objective: The objective of the study was to specify the thickness of Zn and Pb accumulation within the tidemark (TM), a narrow structure between the non-calcified and the calcified articular cartilage. It is considered an active or resting calcification front. This banded structure of the cartilage-bone interface is known to undergo changes in osteoarthritis. Therefore, gaining knowledge about this structure is of interest. Methods: Femoral head samples were collected from patients suffering from various bone diseases, 6 samples have been investigated. Thin bone slices (3μm thick) were measured with high resolution synchrotron micro-X-ray fluorescence (SR micro-XRF) analysis using a beam with dimensions of 500 × 800 nm2. The tidemark region was found in all analyzed samples. The Savitzky-Golay filter was used to smooth the measured imaging data and Kaplan-Meier estimation to gain reliable tidemarks medians for Pb and Zn. To our knowledge this was the first time that these methods have been applied to gain information on histological structures obtained by elemental imaging. Results: The thickness of the Zn and Pb layer ranged from about 3 to 11 μm for Zn and 4 to 14.5 μm for Pb. Our Zn ratios (TM/matrix) were found to be 1.5-3-fold ratio between Zn tidemark values and in mineralized matrix and are similar in all samples. Conclusions: The determined thickness of the layer is much smaller than found in previous measurements with the beam having 20 × 14 μm2 size. The Zn ratios agree with our previous findings.

Journal Keywords: Tidemark; Zinc; Lead; SR micro-XRF; Elemental Imaging

Diamond Keywords: Bone; Osteoarthritis

Subject Areas: Biology and Bio-materials


Instruments: B16-Test Beamline

Added On: 01/06/2021 09:45

Documents:
1-s2.0-S2665913121000455-main.pdf

Discipline Tags:

Non-Communicable Diseases Health & Wellbeing Life Sciences & Biotech

Technical Tags:

Imaging X-ray Fluorescence (XRF)