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Quantitative phase measurements of human cell nuclei using X-ray ptychography

DOI: 10.1107/S1600577521004586 DOI Help

Authors: Jorg Schwenke (University College London; Research Complex at Harwell (RCaH); Lund University) , Mohammed Yusuf (University College London; Research Complex at Harwell (RCaH); Aga Khan University) , Laura A. Shemilt (University College London; Research Complex at Harwell (RCaH)) , Ulrich Wagner (Diamond Light Source) , Atiqa Sajid (Aga Khan University) , Graeme R. Morrison (University College London; Research Complex at Harwell (RCaH)) , Fucai Zhang (University College London) , Aaron Parsons (Diamond Light Source) , Christoph Rau (Diamond Light Source) , Ian K. Robinson (University College London; Research Complex at Harwell (RCaH); Brookhaven National Laboratory)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal Of Synchrotron Radiation , VOL 28

State: Published (Approved)
Published: July 2021

Abstract: The human cell nucleus serves as an important organelle holding the genetic blueprint for life. In this work, X-ray ptychography was applied to assess the masses of human cell nuclei using its unique phase shift information. Measurements were carried out at the I13-1 beamline at the Diamond Light Source that has extremely large transverse coherence properties. The ptychographic diffractive imaging approach allowed imaging of large structures that gave quantitative measurements of the phase shift in 2D projections. In this paper a modified ptychography algorithm that improves the quality of the reconstruction for weak scattering samples is presented. The application of this approach to calculate the mass of several human nuclei is also demonstrated.

Journal Keywords: X-ray; ptychography; nuclei; quantitative phase imaging; synchrotron.

Subject Areas: Biology and Bio-materials, Technique Development

Instruments: I13-1-Coherence

Added On: 25/06/2021 13:55

Discipline Tags:

Technique Development - Life Sciences & Biotech Life Sciences & Biotech

Technical Tags:

Imaging Coherent Diffraction Imaging (CDI) Ptychography