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Phosphorus and sulfur SAD phasing of the nucleic acid-bound DNA-binding domain of interferon regulatory factor 4

DOI: 10.1107/S2053230X21006506 DOI Help

Authors: Alessandro Agnarelli (University of Sussex) , Kamel El Omari (Diamond Light Source; Research Complex at Harwell) , Ramona Duman (Diamond Light Source; Research Complex at Harwell) , Armin Wagner (Diamond Light Source; Research Complex at Harwell) , Erika J. Mancini (University of Sussex)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section F Structural Biology Communications , VOL 77

State: Published (Approved)
Published: July 2021
Diamond Proposal Number(s): 20145

Open Access Open Access

Abstract: Pivotal to the regulation of key cellular processes such as the transcription, replication and repair of DNA, DNA-binding proteins play vital roles in all aspects of genetic activity. The determination of high-quality structures of DNA-binding proteins, particularly those in complexes with DNA, provides crucial insights into the understanding of these processes. The presence in such complexes of phosphate-rich oligonucleotides offers the choice of a rapid method for the routine solution of DNA-binding proteins through the use of long-wavelength beamlines such as I23 at Diamond Light Source. This article reports the use of native intrinsic phosphorus and sulfur single-wavelength anomalous dispersion methods to solve the complex of the DNA-binding domain (DBD) of interferon regulatory factor 4 (IRF4) bound to its interferon-stimulated response element (ISRE). The structure unexpectedly shows three molecules of the IRF4 DBD bound to one ISRE. The sole reliance on native intrinsic anomalous scattering elements that belong to DNA–protein complexes renders the method of general applicability to a large number of such protein complexes that cannot be solved by molecular replacement or by other phasing methods.

Journal Keywords: experimental phasing; native SAD; phosphorus; DNA; DNA-binding proteins; IRF4; interferon regulatory factor 4

Subject Areas: Biology and Bio-materials, Technique Development

Instruments: I04-Macromolecular Crystallography , I23-Long wavelength MX

Added On: 30/06/2021 11:47


Discipline Tags:

Technique Development - Life Sciences & Biotech Structural biology Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX) Long Wavelength Crystallography