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Structure of an H3N2 influenza virus nucleoprotein

DOI: 10.1107/S2053230X2100635X DOI Help

Authors: Michael L. Knight (University of Oxford) , Haitian Fan (University of Oxford) , David L. V. Bauer (Francis Crick Institute) , Jonathan M. Grimes (University of Oxford) , Ervin Fodor (University of Oxford) , Jeremy Keown (University of Oxford)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section F Structural Biology Communications , VOL 77 , PAGES 208 - 214

State: Published (Approved)
Published: July 2021
Diamond Proposal Number(s): 19946

Open Access Open Access

Abstract: Influenza A viruses of the H1N1 and H3N2 subtypes are responsible for seasonal epidemic events. The influenza nucleoprotein (NP) binds to the viral genomic RNA and is essential for its replication. Efforts are under way to produce therapeutics and vaccines targeting the NP. Despite this, no structure of an NP from an H3N2 virus has previously been determined. Here, the structure of the A/Northern Territory/60/1968 (H3N2) influenza virus NP is presented at 2.2 Å resolution. The structure is highly similar to those of the A/WSN/1933 (H1N1) and A/Hong Kong/483/97 (H5N1) NPs. Nonconserved amino acids are widely dispersed both at the sequence and structural levels. A movement of the 73–90 RNA-binding loop is observed to be the key difference between the structure determined here and previous structures. The data presented here increase the understanding of structural conservation amongst influenza NPs and may aid in the design of universal interventions against influenza.

Journal Keywords: influenza; H3N2 influenza virus nucleoprotein; X-ray crystallography; nucleoprotein; RNA-binding protein

Diamond Keywords: Influenza; Viruses

Subject Areas: Biology and Bio-materials, Medicine


Instruments: I24-Microfocus Macromolecular Crystallography

Added On: 07/07/2021 09:12

Documents:
uf5004.pdf

Discipline Tags:

Life Sciences & Biotech Health & Wellbeing Drug Discovery Infectious Diseases Pathogens Structural biology

Technical Tags:

Diffraction Macromolecular Crystallography (MX)