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CryoEM structure of the Nipah virus nucleocapsid assembly
DOI:
10.1371/journal.ppat.1009740
Authors:
De-Sheng
Ker
(University of York)
,
Huw T.
Jenkins
(University of York)
,
Sandra J.
Greive
(University of York)
,
Alfred A.
Antson
(University of York)
Co-authored by industrial partner:
No
Type:
Journal Paper
Journal:
Plos Pathogens
, VOL 17
State:
Published (Approved)
Published:
July 2021
Diamond Proposal Number(s):
19832

Abstract: Nipah and its close relative Hendra are highly pathogenic zoonotic viruses, storing their ssRNA genome in a helical nucleocapsid assembly formed by the N protein, a major viral immunogen. Here, we report the first cryoEM structure for a Henipavirus RNA-bound nucleocapsid assembly, at 3.5 Å resolution. The helical assembly is stabilised by previously undefined N- and C-terminal segments, contributing to subunit-subunit interactions. RNA is wrapped around the nucleocapsid protein assembly with a periodicity of six nucleotides per protomer, in the “3-bases-in, 3-bases-out” conformation, with protein plasticity enabling non-sequence specific interactions. The structure reveals commonalities in RNA binding pockets and in the conformation of bound RNA, not only with members of the Paramyxoviridae family, but also with the evolutionarily distant Filoviridae Ebola virus. Significant structural differences with other Paramyxoviridae members are also observed, particularly in the position and length of the exposed α-helix, residues 123–139, which may serve as a valuable epitope for surveillance and diagnostics.
Journal Keywords: Nucleocapsids; RNA structure; Paramyxoviruses; Protein interactions; Protein structure; Nipah virus; Protein structure comparison; Protein structure determination
Diamond Keywords: Nipah Virus (NiV); Viruses
Subject Areas:
Biology and Bio-materials,
Medicine
Diamond Offline Facilities:
Electron Bio-Imaging Centre (eBIC)
Instruments:
Krios II-Titan Krios II at Diamond
Added On:
21/07/2021 13:30
Documents:
journal.ppat.1009740.pdf
Discipline Tags:
Pathogens
Infectious Diseases
Disease in the Developing World
Health & Wellbeing
Structural biology
Drug Discovery
Life Sciences & Biotech
Technical Tags:
Microscopy
Electron Microscopy (EM)
Cryo Electron Microscopy (Cryo EM)