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Visualising the ionome in resistant and susceptible plant‐pathogen interactions

DOI: 10.1111/tpj.15469 DOI Help

Authors: S. M. Brouwer (Swedish University of Agricultural Sciences) , P. Lindqvist-Reis (Swedish University of Agricultural Sciences; Karlsruhe Institute of Technology) , D. Pergament Persson (University of Copenhagen) , S. Marttila (Swedish University of Agricultural Sciences) , L. Grenville-Briggs (Swedish University of Agricultural Sciences) , E. Andreasson (Swedish University of Agricultural Sciences)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: The Plant Journal

State: Published (Approved)
Published: August 2021
Diamond Proposal Number(s): 24489

Abstract: At the morphological and anatomical level the ionome, or the elemental composition of an organism, is an understudied area of plant biology. In particular, the ionomic responses of plant-pathogen interactions are scarcely described, and there are no studies on immune reactions. In this study we explored two X-ray fluorescence based ionome visualisation methods (benchtop and synchrotron-based micro XRF (µXRF), as well as the quantitative Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES) method, to investigate the changes that occur in the ionome of compatible and incompatible plant-pathogen interactions. We utilised the agronomically important and comprehensively studied interaction between potato, Solanum tuberosum, and the late blight oomycete pathogen, Phytophthora infestans, as an example. We used one late blight susceptible potato cultivar and two resistant transgenic plant lines (only differing from the susceptible cultivar in one or three resistance genes) both in control and P. infestans inoculated conditions. In the lesions from the compatible interaction we observed rearrangements of several elements. This included, a decrease of the mobile macro nutrient potassium (K), and an increase of iron (Fe), and manganese (Mn) in the lesion versus the tissue outside the lesion. Interestingly, we observed distinctly different distribution patterns of accumulation at the site of inoculation in the resistant lines for calcium (Ca), Magnesium, Mn and silicon (Si) compared to the susceptible cultivar. The results reveal different ionomes in diseased plants compared to resistant plants. Our results demonstrate a technical advance and pave the way for deeper studies of the plant-pathogen ionome in the future.

Subject Areas: Biology and Bio-materials

Instruments: I18-Microfocus Spectroscopy

Added On: 19/08/2021 13:33

Discipline Tags:

Plant science Pathogens Agriculture & Fisheries Life Sciences & Biotech

Technical Tags:

Imaging X-ray Fluorescence (XRF)