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X‐ray microtomography reveals a lattice‐like network within aortic elastic lamellae

DOI: 10.1096/fj.202100323RR DOI Help

Authors: Aïcha Ben Zemzem (CNRS UMR7369 MEDyC, University of Reims Champagne-Ardenne) , Aline Genevaux (CNRS UMR7369 MEDyC, University of Reims Champagne-Ardenne) , Amandine Wahart (CNRS UMR7369 MEDyC, University of Reims Champagne-Ardenne) , Andrew J. Bodey (Diamond Light Source) , Sébastien Blaise (CNRS UMR7369 MEDyC, University of Reims Champagne-Ardenne) , Béatrice Romier-Crouzet (CNRS UMR7369 MEDyC, University of Reims Champagne-Ardenne) , Jessica Jonquet (CNRS UMR7369 MEDyC, University of Reims Champagne-Ardenne) , Camille Bour (CNRS UMR7369 MEDyC, University of Reims Champagne-Ardenne) , Rémi Cogranne (Troyes University of Technology, UMR CNRS 6281 ICD/ROSAS/LM2S) , Pierre Beauseroy (Troyes University of Technology, UMR CNRS 6281 ICD/ROSAS/LM2S) , Manuel Dauchez (CNRS UMR7369 MEDyC, University of Reims Champagne-Ardenne) , Michael J. Sherratt (The University of Manchester) , Laurent Debelle (CNRS UMR7369 MEDyC, University of Reims Champagne-Ardenne; The University of Manchester) , Sébastien Almagro (CNRS UMR7369 MEDyC, University of Reims Champagne-Ardenne)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: The Faseb Journal , VOL 35

State: Published (Approved)
Published: October 2021
Diamond Proposal Number(s): 12776

Open Access Open Access

Abstract: The arterial wall consists of three concentric layers: intima, media, and adventitia. Beyond their resident cells, these layers are characterized by an extracellular matrix (ECM), which provides both biochemical and mechanical support. Elastin, the major component of arterial ECM, is present in the medial layer and organized in concentric elastic lamellae that confer resilience to the wall. We explored the arterial wall structures from C57Bl6 (control), db/db (diabetic), and ApoE−/− (atherogenic) mice aged 3 months using synchrotron X-ray computed microtomography on fixed and unstained tissues with a large image field (8 mm3). This approach combined a good resolution (0.83 µm/voxel), large 3D imaging field. and an excellent signal to noise ratio conferred by phase-contrast imaging. We determined from 2D virtual slices that the thickness of intramural ECM structures was comparable between strains but automated image analysis of the 3D arterial volumes revealed a lattice-like network within concentric elastic lamellae. We hypothesize that this network could play a role in arterial mechanics. This work demonstrates that phase-contrast synchrotron X-ray computed microtomography is a powerful technique which to characterize unstained soft tissues.

Subject Areas: Biology and Bio-materials


Instruments: I13-2-Diamond Manchester Imaging

Documents:
fj.202100323RR.pdf

Discipline Tags:

Health & Wellbeing Life Sciences & Biotech

Technical Tags:

Imaging Tomography