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Location dependent lanthanide selectivity engineered into structurally characterized designed coiled coils

DOI: 10.1002/anie.202110500 DOI Help

Authors: Louise N. Slope (University of Birmingham) , Oliver J. Daubney (University of Birmingham) , Hannah Campbell (University of Birmingham) , Scott A. White (University of Birmingham) , Anna Peacock (University of Birmingham)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Angewandte Chemie International Edition

State: Published (Approved)
Published: September 2021
Diamond Proposal Number(s): 14692

Open Access Open Access

Abstract: Herein we report unprecedented location-dependent, size-selective binding to designed lanthanide (Ln 3+ ) sites within miniature protein coiled coil scaffolds. Not only do these engineered sites display unusual Ln 3+ selectivity for moderately large Ln 3+ ions (Nd to Tb), for the first time we demonstrate that selectivity can be location-dependent and can be programmed into the sequence. A 1 nm linear translation of the binding site towards the N-terminus can convert a selective site into a highly promiscuous one. An X-ray crystal structure, the first of a lanthanide binding site within a coiled coil to be reported, coupled with CD studies, reveal the existence of an optimal radius that likely stems from the structural constraints of the coiled coil scaffold. To the best of our knowledge this is the first report of location-dependent metal selectivity within a coiled coil scaffold, as well as the first report of location-dependent Ln 3+ selectivity within a protein.

Journal Keywords: Bioinorganic; Coiled Coils; Lanthanides; Peptide; Protein Design

Subject Areas: Chemistry, Biology and Bio-materials, Engineering

Instruments: I04-1-Macromolecular Crystallography (fixed wavelength)

Added On: 09/09/2021 13:36


Discipline Tags:

Biotechnology Physical Chemistry Biochemistry Chemistry Structural biology Engineering & Technology Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)