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Studying bacterial chemosensory array with CryoEM

DOI: 10.1042/BST20210080 DOI Help

Authors: Zhuan Qin (Wellcome Trust Centre for Human Genetics, University of Oxford) , Peijun Zhang (Wellcome Trust Centre for Human Genetics, University of Oxford; Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Biochemical Society Transactions , VOL 106

State: Published (Approved)
Published: September 2021

Open Access Open Access

Abstract: Bacteria direct their movement in respond to gradients of nutrients and other stimuli in the environment through the chemosensory system. The behavior is mediated by chemosensory arrays that are made up of thousands of proteins to form an organized array near the cell pole. In this review, we briefly introduce the architecture and function of the chemosensory array and its core signaling unit. We describe the in vivo and in vitro systems that have been used for structural studies of chemosensory array by cryoEM, including reconstituted lipid nanodiscs, 2D lipid monolayer arrays, lysed bacterial ghosts, bacterial minicells and native bacteria cells. Lastly, we review recent advances in structural analysis of chemosensory arrays using state-of-the-art cryoEM and cryoET methodologies, focusing on the latest developments and insights with a perspective on current challenges and future directions.

Journal Keywords: bacterial chemotaxis; chemoreceptor array; cryo-electron tomography; cryoEM; in-situ structure; subtomogram averaging

Diamond Keywords: Bacteria

Subject Areas: Biology and Bio-materials


Technical Areas:

Added On: 10/09/2021 08:54

Documents:
bst-2021-0080c.pdf

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Life Sciences & Biotech Structural biology

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