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Balanced lipase interactions for degradation-controlled paclitaxel release from lipid cubic phase formulations

DOI: 10.1016/j.jcis.2021.09.024 DOI Help

Authors: Michele Dully (University of Limerick) , Shayon Bhattacharya (University of Limerick) , Vivek Verma (University of Limerick) , David Murray (COOK Ireland Limited) , Damien Thompson (University of Limerick) , Tewfik Soulimane (University of Limerick) , Sarah P. Hudson (University of Limerick)
Co-authored by industrial partner: Yes

Type: Journal Paper
Journal: Journal Of Colloid And Interface Science , VOL 31

State: Published (Approved)
Published: September 2021
Diamond Proposal Number(s): 24926

Open Access Open Access

Abstract: Lipid cubic phase (LCP) formulations enhance the intestinal solubility and bioavailability of hydrophobic drugs by reducing precipitation and facilitating their mass transport to the intestinal surface for absorption. LCPs with an ester linkage connecting the acyl chain to the glycerol backbone (monoacylglycerols), are susceptible to chemical digestion by several lipolytic enzymes including lipases, accelerating the release of hydrophobic agents from the lipid bilayers of the matrix. Unlike regular enzymes that transform soluble substrates, lipolytic enzymes act at the interface of water and insoluble lipid. Therefore, compounds that bind to this interface can enhance or inhibit the activity of enzymes to varying extent. Here, we explore how the lipolysis rate can be tuned by the interfacial interaction of porcine pancreatic lipase with monoolein LCPs containing a known lipase inhibitor, tetrahydrolipstatin. Release of the Biopharmaceutical Classification System (BCS) class IV drug, paclitaxel, from the inhibitor-modified LCP was examined in the presence of lipase and its effectors colipase and calcium. By combining experimental dynamic digestion studies, thermodynamic measurements and molecular dynamics simulations of the competitive inhibition of lipase by tetrahydrolipstatin, we reveal the role and mode of action of lipase effectors in creating a precisely-balanced degradation-controlled LCP release system for the poorly soluble paclitaxel drug.

Journal Keywords: Lipid cubic phase; pancreatic lipase; tetrahydrolipstatin; colipase; controlled release; paclitaxel; molecular dynamics; predictive modelling; binding energy; density functional theory

Subject Areas: Medicine, Biology and Bio-materials, Chemistry


Instruments: B21-High Throughput SAXS

Added On: 18/09/2021 11:47

Documents:
1-s2.0-S0021979721014818-main.pdf

Discipline Tags:

Drug Delivery Health & Wellbeing Biochemistry Soft condensed matter physics Chemistry Organic Chemistry Life Sciences & Biotech

Technical Tags:

Scattering Small Angle X-ray Scattering (SAXS)