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CryoEM structure of the super-constricted two-start dynamin 1 filament

DOI: 10.1038/s41467-021-25741-x DOI Help

Authors: Jiwei Liu (Wellcome Centre for Human Genetics, University of Oxford) , Frances Joan D. Alvarez (University of Pittsburgh School of Medicine) , Daniel K. Clare (Diamond Light Source) , Jeffrey K. Noel (Electric Ant Lab) , Peijun Zhang (Wellcome Centre for Human Genetics, University of Oxford; University of Pittsburgh School of Medicine; Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Nature Communications , VOL 12

State: Published (Approved)
Published: September 2021
Diamond Proposal Number(s): 14393

Open Access Open Access

Abstract: Dynamin belongs to the large GTPase superfamily, and mediates the fission of vesicles during endocytosis. Dynamin molecules are recruited to the neck of budding vesicles to assemble into a helical collar and to constrict the underlying membrane. Two helical forms were observed: the one-start helix in the constricted state and the two-start helix in the super-constricted state. Here we report the cryoEM structure of a super-constricted two-start dynamin 1 filament at 3.74 Å resolution. The two strands are joined by the conserved GTPase dimeric interface. In comparison with the one-start structure, a rotation around Hinge 1 is observed, essential for communicating the chemical power of the GTPase domain and the mechanical force of the Stalk and PH domain onto the underlying membrane. The Stalk interfaces are well conserved and serve as fulcrums for adapting to changing curvatures. Relative to one-start, small rotations per interface accumulate to bring a drastic change in the helical pitch. Elasticity theory rationalizes the diversity of dynamin helical symmetries and suggests corresponding functional significance.

Journal Keywords: Cryoelectron microscopy; Endocytosis; Membrane fission; Proteins

Subject Areas: Biology and Bio-materials

Diamond Offline Facilities: Electron Bio-Imaging Centre (eBIC)
Instruments: Krios I-Titan Krios I at Diamond

Added On: 18/09/2021 19:54

Documents:
s41467-021-25741-x.pdf

Discipline Tags:

Life Sciences & Biotech Structural biology

Technical Tags:

Microscopy Electron Microscopy (EM) Cryo Electron Microscopy (Cryo EM)