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Molecular mechanism of sugar transport in plants unveiled by structures of glucose/H+ symporter STP10

DOI: 10.1038/s41477-021-00992-0 DOI Help

Authors: Laust Bavnhøj (Aarhus University) , Peter Aasted Paulsen (Aarhus University) , Jose C. Flores-Canales (Aarhus University) , Birgit Schiøtt (Aarhus University) , Bjorn Panyella Pedersen (Aarhus University)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Nature Plants , VOL 4

State: Published (Approved)
Published: September 2021
Diamond Proposal Number(s): 23316 , 17844

Abstract: Sugars are essential sources of energy and carbon and also function as key signalling molecules in plants. Sugar transport proteins (STP) are proton-coupled symporters responsible for uptake of glucose from the apoplast into plant cells. They are integral to organ development in symplastically isolated tissues such as seed, pollen and fruit. Additionally, STPs play a vital role in plant responses to stressors such as dehydration and prevalent fungal infections like rust and mildew. Here we present a structure of Arabidopsis thaliana STP10 in the inward-open conformation at 2.6 Å resolution and a structure of the outward-occluded conformation at improved 1.8 Å resolution, both with glucose and protons bound. The two structures describe key states in the STP transport cycle. Together with molecular dynamics simulations that establish protonation states and biochemical analysis, they pinpoint structural elements, conserved in all STPs, that clarify the basis of proton-to-glucose coupling. These results advance our understanding of monosaccharide uptake, which is essential for plant organ development, and set the stage for bioengineering strategies in crops.

Journal Keywords: Plant molecular biology; Plant sciences

Subject Areas: Biology and Bio-materials, Chemistry

Instruments: I04-Macromolecular Crystallography , I24-Microfocus Macromolecular Crystallography

Other Facilities: BioMAX at MAX IV; DESY-PETRA III

Added On: 28/09/2021 10:04

Discipline Tags:

Plant science Life Sciences & Biotech Agriculture & Fisheries Chemistry Biochemistry

Technical Tags:

Diffraction Macromolecular Crystallography (MX)