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Multi‐scale ensemble properties of the Escherichia coli RNA degradosome

DOI: 10.1111/mmi.14800 DOI Help

Authors: Tom Dendooven (University of Cambridge) , Giulia Paris (University of Cambridge) , Alexander V. Shkumatov (Vlaams Instituut voor Biotechnologie; Vrije Universiteit Brussel) , Md. Saiful Islam (University of Cambridge) , Alister Burt (Diamond Light Source) , Marta A. Kubańska (University of Cambridge) , Tai Yuchen Yang (University of Cambridge) , Steven W. Hardwick (University of Cambridge) , Ben F. Luisi (University of Cambridge)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Molecular Microbiology , VOL 8

State: Published (Approved)
Published: September 2021

Open Access Open Access

Abstract: In organisms from all domains of life, multi-enzyme assemblies play central roles in defining transcript lifetimes and facilitating RNA-mediated regulation of gene expression. An assembly dedicated to such roles, known as the RNA degradosome, is found amongst bacteria from highly diverse lineages. About a fifth of the assembly mass of the degradosome of Escherichia coli and related species is predicted to be intrinsically disordered – a property that has been sustained for over a billion years of bacterial molecular history and stands in marked contrast to the high degree of sequence variation of that same region. Here, we characterize the conformational dynamics of the degradosome using a hybrid structural biology approach that combines solution scattering with ad hoc ensemble modelling, cryo-electron microscopy, and other biophysical methods. The E. coli degradosome can form punctate bodies in vivo that may facilitate its functional activities, and based on our results, we propose an electrostatic switch model to account for the propensity of the degradosome to undergo programmable puncta formation.

Journal Keywords: Enzyme; Bacteria

Diamond Keywords: colloidal polymer; intrinsically disordered protein; post-transcriptional gene regulation; ribonuclease; riboregulation; RNA condensates; RNA metabolism; RNA surveillance; RNase E

Subject Areas: Biology and Bio-materials

Instruments: B21-High Throughput SAXS

Added On: 30/09/2021 11:04


Discipline Tags:

Structural biology Life Sciences & Biotech

Technical Tags:

Scattering Small Angle X-ray Scattering (SAXS)