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Label-free, real-time measurement of metabolism of adherent and suspended single cells by in-cell Fourier Transform Infrared Microspectroscopy

DOI: 10.3390/ijms221910742 DOI Help

Authors: Tommaso Vannocci (UK Dementia Research Institute at The Wohl Institute of King’s College London) , Luca Quaroni (Jagiellonian University) , Antonio De Riso (Evotec (UK) Ltd) , Giulia Milordini (UK Dementia Research Institute at The Wohl Institute of King’s College London) , Magda Wolna (Diamond Light Source) , Gianfelice Cinque (Diamond Light Source) , Annalisa Pastore (UK Dementia Research Institute at The Wohl Institute of King’s College London)
Co-authored by industrial partner: Yes

Type: Journal Paper
Journal: International Journal Of Molecular Sciences , VOL 22

State: Published (Approved)
Published: October 2021
Diamond Proposal Number(s): 18811

Open Access Open Access

Abstract: We used infrared (IR) microscopy to monitor in real-time the metabolic turnover of individual mammalian cells in morphologically different states. By relying on the intrinsic absorption of mid-IR light by molecular components, we could discriminate the metabolism of adherent cells as compared to suspended cells. We identified major biochemical differences between the two cellular states, whereby only adherent cells appeared to rely heavily on glycolytic turnover and lactic fermentation. We also report spectroscopic variations that appear as spectral oscillations in the IR domain, observed only when using synchrotron infrared radiation. We propose that this effect could be used as a reporter of the cellular conditions. Our results are instrumental in establishing IR microscopy as a label-free method for real-time metabolic studies of individual cells in different morphological states, and in more complex cellular ensembles.

Journal Keywords: cellular metabolism; glycolysis; infrared microscopy; synchrotron infrared; cellular adhesion

Subject Areas: Biology and Bio-materials, Chemistry, Technique Development


Instruments: B22-Multimode InfraRed imaging And Microspectroscopy

Added On: 08/10/2021 08:49

Documents:
ijms-22-10742-v3.pdf

Discipline Tags:

Life Sciences & Biotech Technique Development - Life Sciences & Biotech Chemistry Biochemistry

Technical Tags:

Spectroscopy Infrared Spectroscopy Synchtron-based Fourier Transform Infrared Spectroscopy (SR-FTIR)