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Novel in situ multi-level analysis of structural-mechanical relations in a bioinspired polyurethane-based tissue model

DOI: 10.1016/j.mtadv.2021.100184 DOI Help

Authors: Jingyi Mo (University of Surrey) , Nathanael Leung (University of Surrey) , Priyanka Gupta (University of Surrey; University College London) , Bin Zhu (University of Surrey) , Eirini Velliou (University of Surrey; University College London) , Tan Sui (University of Surrey)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Materials Today Advances , VOL 12

State: Published (Approved)
Published: December 2021
Diamond Proposal Number(s): 20046

Open Access Open Access

Abstract: In this manuscript, we elucidated, for the first time, the substructural mechanisms present in our recently developed bioinspired polyurethane-based pancreatic tissue models. Different protein coatings of the model, i.e., collagen and fibronectin were examined. More specifically, analysis took place by combined real-time synchrotron X-ray scattering techniques and confocal laser scanning microscopy, to quantify the structural alteration of uncoated-polyurethane (PU) and protein-coated PU as well as the time-resolved structural reorganisation occurring at the micro-, nano- and lattice length scales during in situ micromechanical testing. We demonstrate that a clear increase of stiffness at the lamellar level following the fibronectin-PU modification, which is linked to the changes in the mechanics of the lamellae and interlamellar cohesion. This multi-level analysis of structural-mechanical relations in this polyurethane-based pancreatic cancer tissue model opens an opportunity in designing mechanically robust cost-effective tissue models not only for fundamental research but also for treatment screening.

Journal Keywords: Bioinspired polyurethane; Surface functionalisation; Synchrotron X-ray scattering techniques; Confocal laser scanning microscopy; In situ micromechanical testing; Pancreatic cancer; Cancer cells; 3D scaffolds; Extracellular matrix (ECM); Collagen; Fibrone

Diamond Keywords: Pancreatic Cancer

Subject Areas: Biology and Bio-materials


Instruments: B16-Test Beamline

Added On: 08/11/2021 08:57

Documents:
1-s2.0-S2590049821000540-main.pdf

Discipline Tags:

Life Sciences & Biotech Health & Wellbeing Cancer Non-Communicable Diseases Materials Science Biomaterials

Technical Tags:

Scattering Small Angle X-ray Scattering (SAXS) Wide Angle X-ray Scattering (WAXS)