A potential new method for vaccines without refrigeration

Authors: Asel Sartbaeva (University of Bath)
Co-authored by industrial partner: No

Type: Diamond Annual Review Highlight

State: Published (Approved)
Published: July 2021
Diamond Proposal Number(s): 14148

Abstract: Almost all vaccines require refrigeration or freezing conditions for storage and transport. A vaccine cold chain has been developed to DA An international team of researchers, led by Dr Asel Sartbaeva, has developed a novel method of making existing vaccines thermally stable so that they will not depend on cold chain distribution. The process uses silica (SiO2) to create layers of inorganic materials around individual vaccine components. This ensilication results in nanoparticles of silica with protein in the middle. The team performed in situ Small Angle X-ray Scattering (SAXS) measurements during ensilication, on Diamond Light Source’s Time-Resolved SAXS & Diffraction beamline (I22). They chose to study tetanus toxin C fragment (TTCF), an inactive component of the tetanus toxin present in the diphtheria, tetanus and pertussis (DTP) vaccine. Their results demonstrated that ensilication maintained the vaccine effect. All biopharmaceuticals have unique functions that require different environments for their operation. For some, lyophilisation (freeze-drying) may be the optimum choice for stabilising and transporting the bioactive compounds. However, this new methodology provides another solution for biopharmaceutical stabilisation and could help to increase vaccine transport and administration all around the world.

Journal Keywords: Vaccine; Thermal stability; SAXS; in vivo; in situ; Tetanus toxoid; Silica; Ensilication; Protein; Protein unfolding; Aggregation; enaturation

Diamond Keywords: Tetanus; Bacteria; Cold Chain Distribution

Subject Areas: Biology and Bio-materials, Medicine

Instruments: I22-Small angle scattering & Diffraction

Other Facilities: ID02 at ESRF

Added On: 26/11/2021 13:29

Discipline Tags:

Vaccines Pathogens Infectious Diseases Health & Wellbeing Life Sciences & Biotech

Technical Tags:

Scattering Small Angle X-ray Scattering (SAXS)