Synchrotron-based nano-X-ray absorption near-edge structure revealing intracellular heterogeneity of iron species in magnetotactic bacteria

DOI: 10.1002/smsc.202100089

Authors: Daniel M. Chevrier (CNRS, CEA, BIAM, Aix-Marseille Université; Max Planck Institute of Colloids and Interfaces) , Elisa Cerda-Donate (Max Planck Institute of Colloids and Interfaces) , Yeseul Park (CNRS, CEA, BIAM, Aix-Marseille Université) , Fernando Cacho-Nerin (Diamond Light Source) , Miguel Gomez-Gonzalez (Diamond Light Source) , René Uebe (University of Bayreuth) , Damien Faivre (CNRS, CEA, BIAM, Aix-Marseille Université; Max Planck Institute of Colloids and Interfaces)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Small Science

State: Published (Approved)
Published: December 2021
Diamond Proposal Number(s): 23693 , 23602

Abstract: Magnetotactic bacteria (MTB) sequester iron from the environment to biomineralize magnetite or greigite nanoparticles in magnetosome organelles, though the necessity of intracellular iron storage for the formation process is still in question. Understanding the role of iron storage would make clear the contribution of MTB in geochemical iron cycling and its potential importance during the biosynthesis of application-relevant magnetic nanoparticles. Herein, how scanning X-ray fluorescence microscopy (SXFM) and nanoscale X-ray absorption near-edge structure (nano-XANES) mapping can spatially and chemically identify intracellular iron species is reported, creating an opportunity to examine the role of iron storage in magnetite biomineralization at the single-cell level. Fe K-edge nano-XANES measurements of Magnetospirillum gryphiswaldense in varied iron media conditions and iron storage capacity reveal a significant quantity of intracellular iron heterogeneities through a distinction between formed magnetosomes and intracellular iron material. This intracellular iron component is found in both early and late stages of biomineralization. The capabilities of nano-XANES in providing an experimental advantage in the multidisciplinary field of biomineralization are highlighted.

Journal Keywords: biomineralization; magnetite; magnetosomes; magnetotactic bacteria; nano-X-ray absorption near-edge structure; single cells; X-ray microscopy

Diamond Keywords: Bacteria; Biomineralisation

Subject Areas: Biology and Bio-materials


Instruments: I14-Hard X-ray Nanoprobe

Added On: 28/12/2021 10:37

Documents:
Small Science - 2021 - Chevrier - Synchrotron%E2%80%90Based Nano%E2%80%90X%E2%80%90Ray Absorption Near%E2%80%90Edge Structure Revealing Intracellular.pdf

Discipline Tags:

Biomaterials Technique Development - Materials Science Materials Science Nanoscience/Nanotechnology

Technical Tags:

Imaging X-ray Fluorescence (XRF)