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Natural polyphenol-containing gels against HSV-1 infection: a comparative study

DOI: 10.3390/nano12020227 DOI Help

Authors: Mariaconcetta Sicurella (University of Ferrara) , Maddalena Sguizzato (University of Ferrara) , Paolo Mariani (Università Politecnica delle Marche) , Alessia Pepe (Università Politecnica delle Marche) , Anna Baldisserotto (University of Ferrara) , Raissa Buzzi (University of Ferrara) , Nicolas Huang (CNRS, Institut Galien Paris-Saclay, Université Paris-Saclay) , Fanny Simelière (CNRS, Institut Galien Paris-Saclay, Université Paris-Saclay) , Sam Burholt (Diamond Light Source) , Peggy Marconi (University of Ferrara) , Elisabetta Esposito (University of Ferrara)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Nanomaterials , VOL 12

State: Published (Approved)
Published: January 2022
Diamond Proposal Number(s): 29797

Open Access Open Access

Abstract: Herpes simplex virus type 1 infection commonly affects many people, causing perioral sores, as well as severe complications including encephalitis in immunocompromised patients. The main pharmacological approach involves synthetic antiviral drugs, among which acyclovir is the golden standard, often leading to resistant virus strains under long-term use. An alternative approach based on antiviral plant-derived compounds, such as quercetin and mangiferin, demonstrated an antiviral potential. In the present study, semisolid forms for cutaneous application of quercetin and mangiferin were designed and evaluated to treat HSV-1 infection. Phosphatidylcholine- and poloxamer-based gels were produced and characterized. Gel physical–chemical aspects were evaluated by rheological measurements and X-ray diffraction, evidencing the different thermoresponsive behaviors and supramolecular organizations of semisolid forms. Quercetin and mangiferin diffusion kinetics were compared in vitro by a Franz cell system, demonstrating the different gel efficacies to restrain the polyphenol diffusion. The capability of gels to control polyphenol antioxidant potential and stability was evaluated, indicating a higher stability and antioxidant activity in the case of quercetin loaded in poloxamer-based gel. Furthermore, a plaque reduction assay, conducted to compare the virucidal effect of quercetin and mangiferin loaded in gels against the HSV-1 KOS strain, demonstrated the suitability of poloxamer-based gel to prolong the polyphenol activity.

Journal Keywords: quercetin; mangiferin; polyphenols; HSV-1; localized drug action; infection control; in vitro diffusion; poloxamer; lecithin; gels

Diamond Keywords: Herpes; Viruses

Subject Areas: Medicine, Chemistry, Biology and Bio-materials

Instruments: DL-SAXS-Offline SAXS and Sample Environment Development

Added On: 17/01/2022 09:22


Discipline Tags:

Drug Delivery Pathogens Infectious Diseases Health & Wellbeing Biochemistry Chemistry Organic Chemistry Life Sciences & Biotech

Technical Tags:

Scattering Small Angle X-ray Scattering (SAXS) Wide Angle X-ray Scattering (WAXS)