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Oxidative desulfurization pathway for complete catabolism of sulfoquinovose by bacteria
Authors:
Mahima
Sharma
(University of York)
,
James P.
Lingford
(The Walter and Eliza Hall Institute of Medical Research; University of Melbourne)
,
Marija
Petricevic
(University of Melbourne)
,
Alexander J. D.
Snow
(University of York)
,
Yunyang
Zhang
(University of Melbourne)
,
Michael A.
Järvå
(The Walter and Eliza Hall Institute of Medical Research; University of Melbourne)
,
Janice W.-Y.
Mui
(University of Melbourne)
,
Nichollas E.
Scott
(University of Melbourne at the Peter Doherty Institute for Infection and Immunity)
,
Eleanor C.
Saunders
(University of Melbourne)
,
Runyu
Mao
(The Walter and Eliza Hall Institute of Medical Research; University of Melbourne)
,
Ruwan
Epa
(University of Melbourne)
,
Bruna M.
Da Silva
(University of Melbourne)
,
Douglas E. V.
Pires
(University of Melbourne)
,
David B.
Ascher
(University of Melbourne)
,
Malcolm J.
Mcconville
(University of Melbourne)
,
Gideon J.
Davies
(University of York)
,
Spencer J.
Williams
(University of Melbourne)
,
Ethan D.
Goddard-Borger
(The Walter and Eliza Hall Institute of Medical Research; University of Melbourne)
Co-authored by industrial partner:
No
Type:
Journal Paper
Journal:
Proceedings Of The National Academy Of Sciences
, VOL 119
State:
Published (Approved)
Published:
January 2022
Diamond Proposal Number(s):
18598

Abstract: Catabolism of sulfoquinovose (SQ; 6-deoxy-6-sulfoglucose), the ubiquitous sulfosugar produced by photosynthetic organisms, is an important component of the biogeochemical carbon and sulfur cycles. Here, we describe a pathway for SQ degradation that involves oxidative desulfurization to release sulfite and enable utilization of the entire carbon skeleton of the sugar to support the growth of the plant pathogen Agrobacterium tumefaciens. SQ or its glycoside sulfoquinovosyl glycerol are imported into the cell by an ATP-binding cassette transporter system with an associated SQ binding protein. A sulfoquinovosidase hydrolyzes the SQ glycoside and the liberated SQ is acted on by a flavin mononucleotide-dependent sulfoquinovose monooxygenase, in concert with an NADH-dependent flavin reductase, to release sulfite and 6-oxo-glucose. An NAD(P)H-dependent oxidoreductase reduces the 6-oxo-glucose to glucose, enabling entry into primary metabolic pathways. Structural and biochemical studies provide detailed insights into the recognition of key metabolites by proteins in this pathway. Bioinformatic analyses reveal that the sulfoquinovose monooxygenase pathway is distributed across Alpha- and Betaproteobacteria and is especially prevalent within the Rhizobiales order. This strategy for SQ catabolism is distinct from previously described pathways because it enables the complete utilization of all carbons within SQ by a single organism with concomitant production of inorganic sulfite.
Journal Keywords: carbohydrate metabolism; sulfur cycle; oxidative desulfurization
Diamond Keywords: Bacteria
Subject Areas:
Biology and Bio-materials,
Chemistry
Instruments:
I04-1-Macromolecular Crystallography (fixed wavelength)
,
I04-Macromolecular Crystallography
,
I24-Microfocus Macromolecular Crystallography
Other Facilities: MX-2 at Australian Synchrotron
Added On:
02/02/2022 09:38
Documents:
e2116022119.full.pdf
Discipline Tags:
Plant science
Pathogens
Biochemistry
Chemistry
Structural biology
Life Sciences & Biotech
Technical Tags:
Diffraction
Macromolecular Crystallography (MX)