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Oxidative desulfurization pathway for complete catabolism of sulfoquinovose by bacteria

DOI: 10.1073/pnas.2116022119 DOI Help

Authors: Mahima Sharma (University of York) , James P. Lingford (The Walter and Eliza Hall Institute of Medical Research; University of Melbourne) , Marija Petricevic (University of Melbourne) , Alexander J. D. Snow (University of York) , Yunyang Zhang (University of Melbourne) , Michael A. Järvå (The Walter and Eliza Hall Institute of Medical Research; University of Melbourne) , Janice W.-Y. Mui (University of Melbourne) , Nichollas E. Scott (University of Melbourne at the Peter Doherty Institute for Infection and Immunity) , Eleanor C. Saunders (University of Melbourne) , Runyu Mao (The Walter and Eliza Hall Institute of Medical Research; University of Melbourne) , Ruwan Epa (University of Melbourne) , Bruna M. Da Silva (University of Melbourne) , Douglas E. V. Pires (University of Melbourne) , David B. Ascher (University of Melbourne) , Malcolm J. Mcconville (University of Melbourne) , Gideon J. Davies (University of York) , Spencer J. Williams (University of Melbourne) , Ethan D. Goddard-Borger (The Walter and Eliza Hall Institute of Medical Research; University of Melbourne)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Proceedings Of The National Academy Of Sciences , VOL 119

State: Published (Approved)
Published: January 2022
Diamond Proposal Number(s): 18598

Open Access Open Access

Abstract: Catabolism of sulfoquinovose (SQ; 6-deoxy-6-sulfoglucose), the ubiquitous sulfosugar produced by photosynthetic organisms, is an important component of the biogeochemical carbon and sulfur cycles. Here, we describe a pathway for SQ degradation that involves oxidative desulfurization to release sulfite and enable utilization of the entire carbon skeleton of the sugar to support the growth of the plant pathogen Agrobacterium tumefaciens. SQ or its glycoside sulfoquinovosyl glycerol are imported into the cell by an ATP-binding cassette transporter system with an associated SQ binding protein. A sulfoquinovosidase hydrolyzes the SQ glycoside and the liberated SQ is acted on by a flavin mononucleotide-dependent sulfoquinovose monooxygenase, in concert with an NADH-dependent flavin reductase, to release sulfite and 6-oxo-glucose. An NAD(P)H-dependent oxidoreductase reduces the 6-oxo-glucose to glucose, enabling entry into primary metabolic pathways. Structural and biochemical studies provide detailed insights into the recognition of key metabolites by proteins in this pathway. Bioinformatic analyses reveal that the sulfoquinovose monooxygenase pathway is distributed across Alpha- and Betaproteobacteria and is especially prevalent within the Rhizobiales order. This strategy for SQ catabolism is distinct from previously described pathways because it enables the complete utilization of all carbons within SQ by a single organism with concomitant production of inorganic sulfite.

Journal Keywords: carbohydrate metabolism; sulfur cycle; oxidative desulfurization

Diamond Keywords: Bacteria

Subject Areas: Biology and Bio-materials, Chemistry


Instruments: I04-1-Macromolecular Crystallography (fixed wavelength) , I04-Macromolecular Crystallography , I24-Microfocus Macromolecular Crystallography

Other Facilities: MX-2 at Australian Synchrotron

Added On: 02/02/2022 09:38

Documents:
e2116022119.full.pdf

Discipline Tags:

Plant science Pathogens Biochemistry Chemistry Structural biology Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)