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2.4-Å structure of the double-ring Gemmatimonas phototrophica photosystem

DOI: 10.1126/sciadv.abk3139 DOI Help

Authors: Pu Qian (Thermo Fisher Scientific; University of Sheffield) , Alastair T. Gardiner (Institute of Microbiology, Czech Academy of Sciences) , Ivana Šímová (University of South Bohemia) , Katerina Naydenova (MRC Laboratory of Molecular Biology) , Tristan I. Croll (University of Cambridge) , Philip J. Jackson (University of Sheffield) , Nupur Nupur (Institute of Microbiology, Czech Academy of Sciences) , Miroslav Kloz (Institute of Physics of the Czech Academy of Sciences) , Petra Čubáková (Institute of Physics of the Czech Academy of Sciences) , Marek Kuzma (Institute of Microbiology, Czech Academy of Sciences) , Yonghui Zeng (University of Copenhagen) , Pablo Castro-Hartmann (Thermo Fisher Scientific) , Bart Van Knippenberg (Thermo Fisher Scientific) , Kenneth N. Goldie (University of Basel) , David Kaftan (Institute of Microbiology, Czech Academy of Sciences) , Pavel Hrouzek (Institute of Microbiology, Czech Academy of Sciences) , Jan Hájek (Institute of Microbiology, Czech Academy of Sciences) , Jon Agirre (University of York) , C. Alistair Siebert (Diamond Light Source) , David Bína (University of South Bohemia) , Kasim Sader (Thermo Fisher Scientific) , Henning Stahlberg (EPFL) , Roman Sobotka (Institute of Microbiology, Czech Academy of Sciences; University of South Bohemia) , Christopher J. Russo (MRC Laboratory of Molecular Biology) , Tomáš Polívka (University of South Bohemia) , C. Neil Hunter (University of Sheffield) , Michal Koblížek (Institute of Microbiology, Czech Academy of Sciences)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Science Advances , VOL 8

State: Published (Approved)
Published: February 2022
Diamond Proposal Number(s): 29785

Open Access Open Access

Abstract: Phototrophic Gemmatimonadetes evolved the ability to use solar energy following horizontal transfer of photosynthesis-related genes from an ancient phototrophic proteobacterium. The electron cryo-microscopy structure of the Gemmatimonas phototrophica photosystem at 2.4 Å reveals a unique, double-ring complex. Two unique membrane-extrinsic polypeptides, RC-S and RC-U, hold the central type 2 reaction center (RC) within an inner 16-subunit light-harvesting 1 (LH1) ring, which is encircled by an outer 24-subunit antenna ring (LHh) that adds light-gathering capacity. Femtosecond kinetics reveal the flow of energy within the RC-dLH complex, from the outer LHh ring to LH1 and then to the RC. This structural and functional study shows that G. phototrophica has independently evolved its own compact, robust, and highly effective architecture for harvesting and trapping solar energy.

Diamond Keywords: Photosynthesis; Bacteria

Subject Areas: Biology and Bio-materials

Diamond Offline Facilities: Electron Bio-Imaging Centre (eBIC)
Instruments: Krios I-Titan Krios I at Diamond

Added On: 21/02/2022 11:46

Discipline Tags:

Structural biology Biophysics Life Sciences & Biotech

Technical Tags:

Microscopy Electron Microscopy (EM) Cryo Electron Microscopy (Cryo EM)