Contrasting modes of new world arenavirus neutralization by immunization-elicited monoclonal antibodies

DOI: 10.1128/mbio.02650-21

Authors: Weng M. Ng (Wellcome Centre for Human Genetics, University of Oxford) , Mehmet Sahin (University of Basel) , Stefanie A. Krumm (Kings College London) , Jeffrey Seow (Kings College London) , Antra Zeltina (Wellcome Centre for Human Genetics, University of Oxford) , Karl Harlos (Wellcome Centre for Human Genetics, University of Oxford) , Guido C. Paesen (Wellcome Centre for Human Genetics, University of Oxford) , Daniel D. Pinschewer (University of Basel) , Katie J. Doores (Kings College London) , Thomas A. Bowden (Wellcome Centre for Human Genetics, University of Oxford)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Mbio

State: Published (Approved)
Published: March 2022
Diamond Proposal Number(s): 14744 , 19946

Abstract: Transmission of the New World hemorrhagic fever arenaviruses Junín virus (JUNV) and Machupo virus (MACV) to humans is facilitated, in part, by the interaction between the arenavirus GP1 glycoprotein and the human transferrin receptor 1 (hTfR1). We utilize a mouse model of live-attenuated immunization with envelope exchange viruses to isolate neutralizing monoclonal antibodies (NAbs) specific to JUNV GP1 and MACV GP1. Structures of two NAbs, termed JUN1 and MAC1, demonstrate that they neutralize through disruption of hTfR1 recognition. JUN1 utilizes a binding mode common to all characterized infection- and vaccine-elicited JUNV-specific NAbs, which involves mimicking hTfR1 binding through the insertion of a tyrosine into the receptor-binding site. In contrast, MAC1 undergoes a tyrosine-mediated mode of antigen recognition distinct from that used by the reported anti-JUNV NAbs and the only other characterized anti-MACV NAb. These data reveal the varied modes of GP1-specific recognition among New World arenaviruses by the antibody-mediated immune response.

Diamond Keywords: Argentine Hemorrhagic Fever (AHF); Bolivian Hemorrhagic Fever (BHF); Viruses

Subject Areas: Biology and Bio-materials, Medicine


Instruments: I03-Macromolecular Crystallography , I04-Macromolecular Crystallography

Added On: 29/03/2022 08:59

Documents:
mbio.02650-21.pdf

Discipline Tags:

Pathogens Infectious Diseases Health & Wellbeing Structural biology Drug Discovery Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)