The interaction of styrene maleic acid copolymers with phospholipids in langmuir monolayers, vesicles and nanodiscs; a structural study

DOI: 10.1016/j.jcis.2022.03.102

Authors: Stephen C. I. Hall (University of Birmingham; Diamond Light Source; ISIS Neutron and Muon Source) , Cecilia Tognoloni (University of Bath) , Richard A. Campbell (Institut Laue-Langevin; University of Manchester) , Joanna Richens (University of Nottingham) , Paul O'Shea (University of Nottingham; Lancaster University) , Ann E. Terry (Lund University) , Gareth J. Price (University of Bath) , Tim R. Dafforn (University of Birmingham) , Karen J. Edler (University of Bath) , Thomas Arnold (Diamond Light Source; ISIS Neutron and Muon Source; University of Bath; European Spallation Source ERIC)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal Of Colloid And Interface Science , VOL 8

State: Published (Approved)
Published: March 2022

Abstract: Hypothesis: Self-assembly of amphipathic styrene maleic acid copolymers with phospholipids in aqueous solution results in the formation of ‘nanodiscs’ containing a planar segment of phospholipid bilayer encapsulated by a polymer belt. Recently, studies have reported that lipids rapidly exchange between both nanodiscs in solution and external sources of lipids. Outstanding questions remain regarding details of polymer-lipid interactions, factors influencing lipid exchange and structural effects of such exchange processes. Here, the dynamic behaviour of nanodiscs is investigated, specifically the role of membrane charge and polymer chemistry. Experiments: Two model systems are investigated: fluorescently labelled phospholipid vesicles, and Langmuir monolayers of phospholipids. Using fluorescence spectroscopy and time-resolved neutron reflectometry, the membrane potential, monolayer structure and composition are monitored with respect to time upon polymer and nanodisc interactions. Findings: In the presence of external lipids, polymer chains embed throughout lipid membranes, the extent of which is governed by the net membrane charge. Nanodiscs stabilised by three different polymers will all exchange lipids and polymer with monolayers to differing extents, related to the properties of the stabilising polymer belt. These results demonstrate the dynamic nature of nanodiscs which interact with the local environment and are likely to deposit both lipids and polymer at all stages of use.

Journal Keywords: Langmuir monolayer; neutron reflectometry; nanodisc; Lipid Exchange; polymer; Styrene Maleic Acid (SMA); Styrene Maleic Acid Lipid Particle (SMALP); Reversible, Addition−Fragmentation Chain-Transfer (RAFT); Adsorption

Subject Areas: Chemistry, Materials, Biology and Bio-materials

Facility: FIGARO at Institut Laue-Langevin

Added On: 06/04/2022 09:49

Documents:
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Discipline Tags:

Biotechnology Biochemistry Chemistry Materials Science Engineering & Technology Nanoscience/Nanotechnology Polymer Science Life Sciences & Biotech

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