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X-ray absorption spectroscopy of copper and iron in sheep digesta

DOI: 10.1016/j.jtemb.2022.126987 DOI Help

Authors: A. H. Clarkson (University of Nottingham) , N. R. Kendall (University of Nottingham)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal Of Trace Elements In Medicine And Biology , VOL 72

State: Published (Approved)
Published: July 2022
Diamond Proposal Number(s): 17053

Abstract: Background: The bioavailable supply of copper to ruminants has long been problematic. Complexities in supply exist due to interactions with other dietary elements in the rumen, most notably with iron or molybdenum in combination with sulphur, which can result in copper binding preventing its absorption. The molybdenum-sulphur-copper interaction has been extensively studied over the years. However, very little is known about the iron-sulphur-copper interaction, especially its mode of action in the gastrointestinal tract. Methods In the present work digesta from the rumen and jejunum of sheep fed a high copper, sulphur and iron diet was analysed using X-ray absorption spectroscopy (XAS). Results: X-ray absorption fine structure (XAFS) and X-ray absorption near edge structure (XANES) indicated that all of the copper and iron had changed in bonding in the rumen and that the oxidation state of the elements had been reduced into a mix of Fe2+ & Fe3+ and Cu+ with some Cu0. Conclusion: The copper compounds were most likely to be thiol co-ordinated in line with Cu+ chemistry. Changes to the copper compounds took place in the jejunum, although thiols were still highly favoured the possible existence of a copper-iron-sulphur complex which also included oxygen and chloride was also observed. This possibly has some resemblance to the crystal structure of bornite.

Journal Keywords: Ruminant; Copper; Iron antagonism; XAS; Supplementation

Subject Areas: Biology and Bio-materials, Chemistry

Instruments: I20-Scanning-X-ray spectroscopy (XAS/XES)

Added On: 04/05/2022 08:38

Discipline Tags:

Biochemistry Chemistry Life Sciences & Biotech

Technical Tags:

Spectroscopy X-ray Absorption Spectroscopy (XAS) X-ray Absorption Near Edge Structure (XANES)