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Functional and multiscale 3D structural investigation of brain tissue through correlative in vivo physiology, synchrotron microtomography and volume electron microscopy

DOI: 10.1038/s41467-022-30199-6 DOI Help

Authors: Carles Bosch (The Francis Crick Institute (Midland Road)) , Tobias Ackels (The Francis Crick Institute; University College London) , Alexandra Pacureanu (The Francis Crick Institute; University College London) , Yuxin Zhang (The Francis Crick Institute; University College London) , Christopher J. Peddie (The Francis Crick Institute) , Manuel Berning (Max Planck Institute for Brain Research; Scalable minds GmbH) , Norman Rzepka (Scalable minds GmbH) , Marie-Christine Zdora (University College London,; Diamond Light Source; University of Southampton) , Isabell Whiteley (The Francis Crick Institute; University College London) , Malte Storm (Diamond Light Source; Helmholtz-Zentrum Hereon) , Anne Bonnin (Paul Scherrer Institute) , Christoph Rau (Diamond Light Source) , Troy Margrie (University College London) , Lucy Collinson (The Francis Crick Institute) , Andreas T. Schaefer (The Francis Crick Institute; University College London)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Nature Communications , VOL 13

State: Published (Approved)
Published: May 2022
Diamond Proposal Number(s): 20274

Open Access Open Access

Abstract: Understanding the function of biological tissues requires a coordinated study of physiology and structure, exploring volumes that contain complete functional units at a detail that resolves the relevant features. Here, we introduce an approach to address this challenge: Mouse brain tissue sections containing a region where function was recorded using in vivo 2-photon calcium imaging were stained, dehydrated, resin-embedded and imaged with synchrotron X-ray computed tomography with propagation-based phase contrast (SXRT). SXRT provided context at subcellular detail, and could be followed by targeted acquisition of multiple volumes using serial block-face electron microscopy (SBEM). In the olfactory bulb, combining SXRT and SBEM enabled disambiguation of in vivo-assigned regions of interest. In the hippocampus, we found that superficial pyramidal neurons in CA1a displayed a larger density of spine apparati than deeper ones. Altogether, this approach can enable a functional and structural investigation of subcellular features in the context of cells and tissues.

Journal Keywords: Neural circuits; Olfactory bulb; Spine structure

Subject Areas: Technique Development, Biology and Bio-materials

Instruments: I13-2-Diamond Manchester Imaging

Other Facilities: TOMCAT at Swiss Light Source; ID16A, ID19 at ESRF

Added On: 27/05/2022 14:12


Discipline Tags:

Health & Wellbeing Technique Development - Life Sciences & Biotech Neurology Life Sciences & Biotech

Technical Tags:

Imaging Tomography