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3D-printed SAXS chamber for controlled in situ dialysis and optical characterization

DOI: 10.1107/S1600577522005136 DOI Help

Authors: Tamara Ehm (Tel Aviv University; Ludwig- Maximilians-Universität München) , Julian Philipp (Ludwig-Maximilians-Universität) , Martin Barkey (Ludwig-Maximilians-Universität) , Martina Ober (Ludwig-Maximilians-Universität) , Achim Theo Brinkop (Ludwig-Maximilians-Universität) , David Simml (Ludwig-Maximilians-Universität) , Miriam Von Westphalen (Ludwig-Maximilians-Universität) , Bert Nickel (Ludwig-Maximilians-Universität) , Roy Beck (Tel Aviv University) , Joachim O. Rädler (Ludwig-Maximilians-Universität)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal Of Synchrotron Radiation , VOL 29

State: Published (Approved)
Published: July 2022
Diamond Proposal Number(s): 24693 , 21971

Open Access Open Access

Abstract: 3D printing changes the scope of how samples can be mounted for small-angle X-ray scattering (SAXS). In this paper a 3D-printed X-ray chamber, which allows for in situ exchange of buffer and in situ optical transmission spectroscopy, is presented. The chamber is made of cyclic olefin copolymers (COC), including COC X-ray windows providing ultra-low SAXS background. The design integrates a membrane insert for in situ dialysis of the 100 µl sample volume against a reservoir, which enables measurements of the same sample under multiple conditions using an in-house X-ray setup equipped with a 17.4 keV molybdenum source. The design's capabilities are demonstrated by measuring reversible structural changes in lipid and polymer systems as a function of salt concentration and pH. In the same chambers optical light transmission spectroscopy was carried out measuring the optical turbidity of the mesophases and local pH values using pH-responsive dyes. Microfluidic exchange and optical spectroscopy combined with in situ X-ray scattering enables vast applications for the study of responsive materials.

Journal Keywords: cyclic olefin copolymer; 3D printing; in situ dialysis; in-house measurements; small-angle X-ray scattering

Diamond Keywords: Additive Manufacturing

Subject Areas: Materials

Instruments: B21-High Throughput SAXS

Other Facilities: SIBYLS 12.3.1 at Advanced Light Source

Added On: 30/05/2022 09:05

Journal of Synchrotron Radiation - 2022 - Ehm - 3D%E2%80%90printed SAXS chamber for controlled in situ dialysis and optical.pdf

Discipline Tags:

Materials Science

Technical Tags:

Scattering Small Angle X-ray Scattering (SAXS)