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Sulfated glycan recognition by carbohydrate sulfatases of the human gut microbiota

DOI: 10.1038/s41589-022-01039-x DOI Help

Authors: Ana S. Luis (University of Michigan; University of Gothenburg) , Arnaud Basle (Newcastle University) , Dominic P. Byrne (University of Liverpool) , Gareth S. A. Wright (University of Liverpool) , James A. London (University of Liverpool) , Chunsheng Jin (University of Gothenburg) , Niclas G. Karlsson (University of Gothenburg; Oslo Metropolitan University) , Gunnar C. Hansson (University of Gothenburg) , Patrick A. Eyers (University of Liverpool) , Mirjam Czjzek (Sorbonne Université, Univ Paris 06, CNRS, UMR 8227) , Tristan Barbeyron (Sorbonne Université, Univ Paris 06, CNRS, UMR 8227) , Edwin A. Yates (University of Liverpool) , Eric C. Martens (University of Michigan) , Alan Cartmell (University of Liverpool)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Nature Chemical Biology , VOL 19

State: Published (Approved)
Published: June 2022
Diamond Proposal Number(s): 21970 , 18598

Abstract: Sulfated glycans are ubiquitous nutrient sources for microbial communities that have coevolved with eukaryotic hosts. Bacteria metabolize sulfated glycans by deploying carbohydrate sulfatases that remove sulfate esters. Despite the biological importance of sulfatases, the mechanisms underlying their ability to recognize their glycan substrate remain poorly understood. Here, we use structural biology to determine how sulfatases from the human gut microbiota recognize sulfated glycans. We reveal seven new carbohydrate sulfatase structures spanning four S1 sulfatase subfamilies. Structures of S1_16 and S1_46 represent novel structures of these subfamilies. Structures of S1_11 and S1_15 demonstrate how non-conserved regions of the protein drive specificity toward related but distinct glycan targets. Collectively, these data reveal that carbohydrate sulfatases are highly selective for the glycan component of their substrate. These data provide new approaches for probing sulfated glycan metabolism while revealing the roles carbohydrate sulfatases play in host glycan catabolism.

Diamond Keywords: Gut Microbiota; Bacteria

Subject Areas: Biology and Bio-materials, Chemistry

Instruments: I03-Macromolecular Crystallography , I04-1-Macromolecular Crystallography (fixed wavelength) , I04-Macromolecular Crystallography , I24-Microfocus Macromolecular Crystallography

Other Facilities: PROXIMA_1 at SOLEIL

Added On: 20/06/2022 11:21

Discipline Tags:

Health & Wellbeing Biochemistry Chemistry Structural biology Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)