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Structural basis of ligand specificity in the human pentraxins, C-reactive protein and serum amyloid P component

DOI: 10.1002/jmr.1090 DOI Help

Authors: Halina Mikolajek (University College London) , Simon E. Kolstoe (University College London) , Valerie E. Pye (Birkbeck College, London) , Palma Mangione (University College London) , Mark Pepys (University College London) , Stephen P. Wood (University College London)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal Of Molecular Recognition , VOL 24 (2) , PAGES 371-377

State: Published (Approved)
Published: February 2011

Abstract: The normal physiological roles of the phylogenetically conserved human plasma proteins C-reactive protein (CRP) and serum amyloid P component (SAP) are not known. Novel drugs targeting their ligand specificities are in clinical development as both proteins have significant pathophysiological effects, SAP in promoting amyloidosis and CRP in exacerbating ischemic injury. Both proteins bind to phosphoethanolamine and we show here that, under physiological conditions, phosphoethanolamine is bound with higher affinity by human SAP than by human CRP. An explanation is provided by X-ray crystal structures that show SAP residue Tyr74 allowing additional hydrophobic protein–ligand interactions compared with the equivalent Thr76 of CRP. Docking simulations show many more low energy positions for phosphoethanolamine bound by CRP than by SAP and are consistent with the crystallographic and functional binding results. These fundamental observations on structure–activity relationships will aid the design of improved pentraxin targeting drugs.

Subject Areas: Biology and Bio-materials, Medicine

Instruments: I02-Macromolecular Crystallography

Other Facilities: ID29, ID14–2 at ESRF

Added On: 02/03/2011 09:43

Discipline Tags:

Health & Wellbeing Structural biology Drug Discovery Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)